Department of Microbiology, Dankook University, Cheonan 330-714, Republic of Korea.
J Microbiol. 2009 Dec;47(6):716-20. doi: 10.1007/s12275-009-0126-6. Epub 2010 Feb 4.
A pink-pigmented bacterium, designated SW08-7(T) was isolated from the drinking water of a water purifier. Cells were Gram-negative, rod-shaped, strictly aerobic, and non-spore-forming. It grew optimally at 25 degrees C, pH 6 approximately 7. Phylogenese analysis based on 16S rRNA gene sequence showed that strain SW08-7(T) belongs to the genus Methylobacterium. The highest 16S rRNA gene sequence similarities were found to Methylobacterium mesophilicum JCM 2829(T) (96.9%), Methylobacterium brachiatum B0021(T) (96.9%), Methylobacterium phyllosphaerae CBMB27(T) (96.6%), Methylobacterium radiotolerans JCM 2831(T) (96.6%), and Methylobacterium hispanicum GP34(T) (96.5%). DNA-DNA hybridization experiment revealed low-level (28.5%) of DNA-DNA relatedness between strain SW08-7(T) and Methylobacterium hispanicum. The genomic DNA G+C content was 68.9 mol% and the major isoprenoid quinone was Q-10. The major cellular fatty acid of strain SW08-7(T) was C(18:1) omega7c (79.8+/-2.1%). Results of phylogenetic, phenotypic, and biochemical analyses revealed that strain SW08-7(T) could be classified as representing a novel species of genus Methylobacterium, for which the name Methylobacterium dankookense sp. nov. is proposed. The type strain is SW08-7(T) (=KCTC 22512(T) =DSM 22415(1)).
一株粉红色着色的细菌，SW08-7(T)，从饮水机的饮用水中分离出来。细胞革兰氏阴性，杆状，严格需氧，不产芽孢。最适生长温度为 25 摄氏度，pH 值为 6 左右 7. 根据 16S rRNA 基因序列进行的系统发育分析表明，菌株 SW08-7(T)属于甲基杆菌属。最高的 16S rRNA 基因序列相似度为中温甲基杆菌 JCM 2829(T) (96.9%)，Brachiatum Methylobacterium B0021(T) (96.9%)，Methylobacterium phyllosphaerae CBMB27(T) (96.6%)，Methylobacterium radiotolerans JCM 2831(T) (96.6%)，和 Methylobacterium hispanicum GP34(T) (96.5%)。DNA-DNA 杂交实验表明，菌株 SW08-7(T)与 Methylobacterium hispanicum 之间的 DNA-DNA 相关性较低(28.5%)。基因组 DNA G+C 含量为 68.9mol%，主要异戊二烯醌为 Q-10。菌株 SW08-7(T)的主要细胞脂肪酸为 C(18:1)ω7c (79.8+/-2.1%)。系统发育、表型和生化分析的结果表明，菌株 SW08-7(T)可归类为代表甲基杆菌属的一个新种，建议将其命名为 Methylobacterium dankookense sp. nov.。模式株为 SW08-7(T)=KCTC 22512(T)=DSM 22415(1)。
