利用 11 个基因座 PCR 多重扩增检测法鉴定发现死狼的遗传特征和性别。

Genetic profiles and sex identification of found-dead wolves determined by the use of an 11-loci PCR multiplex.

机构信息

Instituto de Patologia e Imunologia da Universidade do Porto, Porto, Portugal.

出版信息

Forensic Sci Int Genet. 2010 Feb;4(2):68-72. doi: 10.1016/j.fsigen.2009.05.003. Epub 2009 Jun 23.

DOI:10.1016/j.fsigen.2009.05.003

PMID:20129463

Abstract

A previously described canine-specific 9-STR multiplex, now including two markers for sex determination, was tested for the genotyping of 23 wolves from Northern and Central Portugal. The samples were collected at necropsies and presented varying states of preservation. Complete profiles were obtained in 74% of the samples, partial profiles in 22% and one completely null profile. This survey revealed 15 alleles not previously described in dogs, distributed among 6 STR loci. It is shown that this genotyping system, previously tested in domestic dogs, can be reliably used for obtaining complete genetic profiles in wolves with a matching probability of 2.45 x 10(-9) and compatible sex identification, even in sub-optimal samples. Moreover, a population structure analysis using the observed genotypes revealed that this multiplexed 11-loci panel may potentially be used for discriminating between wolves and dogs.

摘要

先前描述的一种犬特异性 9-STR 多重扩增试剂盒，现包括两个用于性别鉴定的标记物，用于对来自葡萄牙北部和中部的 23 只狼进行基因分型。这些样本是在尸检时收集的，保存状态各不相同。74%的样本获得了完整的图谱，22%的样本获得了部分图谱，还有一个完全缺失的图谱。这项调查显示了在犬科动物中以前没有描述过的 15 个等位基因，分布在 6 个 STR 基因座上。结果表明，该基因分型系统在之前已在犬科动物中进行了测试，即使在次优样本中，也可以可靠地用于获得狼的完整遗传图谱，其匹配概率为 2.45 x 10(-9)，并且可以兼容性别鉴定。此外，使用观察到的基因型进行的种群结构分析表明，这种 11 个基因座的多重扩增试剂盒可能有潜力用于区分狼和狗。