Böttger T, Störkel S, Stöckle M, Wahl W, Jugenheimer M, Effenberger-Kim O, Vinh T, Junginger T
Department of General and Abdominal Surgery, Johannes Gutenberg-University Mainz, Germany.
Cancer. 1991 May 1;67(9):2290-4. doi: 10.1002/1097-0142(19910501)67:9<2290::aid-cncr2820670914>3.0.co;2-a.
In 45 patients who underwent an esophagus resection due to a squamous cell carcinoma, in addition to the TNM classification and usual morphologic criteria, the paraffin-embedded material underwent deparaffinization, was rehydrated, and was mechanically and enzymatically processed into a single-cell solution. For evaluating the DNA histogram this was analyzed with the help of automatic single-cell cytophotometric study. The method, contrary to that of flow cytometric study, allows for the selective analysis of tumor cells due to the electronically, previously given selection criteria, whereas artifacts, stroma, and infection cells remain excluded from analysis. The multivariate analysis shows that the prognosis is only correlated with DNA content of the tumor cells. Patients with diploid tumors had a median survival time of 32 months as compared with the 22 months of patients with hypotriploid tumors, and 6.5 months with hypertriploid tumors. DNA cytometric analysis should be included when diagnosing patients with esophagus carcinoma.
在45例因鳞状细胞癌接受食管切除术的患者中，除了TNM分类和常规形态学标准外，对石蜡包埋材料进行脱蜡、复水，并通过机械和酶处理制成单细胞悬液。为评估DNA直方图，借助自动单细胞细胞光度测定研究对其进行分析。与流式细胞术研究方法不同，该方法由于预先设定的电子选择标准，允许对肿瘤细胞进行选择性分析，而伪像、基质和感染细胞则被排除在分析之外。多变量分析表明，预后仅与肿瘤细胞的DNA含量相关。二倍体肿瘤患者的中位生存时间为32个月，相比之下，亚三倍体肿瘤患者为22个月，超三倍体肿瘤患者为6.5个月。在诊断食管癌患者时应纳入DNA细胞计量分析。
