Howard Hughes Medical Institute, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Proc Natl Acad Sci U S A. 2010 Feb 23;107(8):3558-63. doi: 10.1073/pnas.0910172106. Epub 2010 Feb 3.
Although adipose tissue is an expandable and readily attainable source of proliferating, multipotent stem cells, its potential for use in regenerative medicine has not been extensively explored. Here we report that adult human and mouse adipose-derived stem cells can be reprogrammed to induced pluripotent stem (iPS) cells with substantially higher efficiencies than those reported for human and mouse fibroblasts. Unexpectedly, both human and mouse iPS cells can be obtained in feeder-free conditions. We discovered that adipose-derived stem cells intrinsically express high levels of pluripotency factors such as basic FGF, TGFbeta, fibronectin, and vitronectin and can serve as feeders for both autologous and heterologous pluripotent cells. These results demonstrate a great potential for adipose-derived cells in regenerative therapeutics and as a model for studying the molecular mechanisms of feeder-free iPS generation and maintenance.
虽然脂肪组织是一种可扩展且易于获得的增殖多能干细胞来源，但它在再生医学中的应用潜力尚未得到广泛探索。在这里，我们报告称，成年人类和小鼠脂肪来源的干细胞可以被重编程为诱导多能干细胞（iPS 细胞），其效率明显高于报道的人类和小鼠成纤维细胞。出乎意料的是，在无饲养层条件下可以获得人和小鼠的 iPS 细胞。我们发现，脂肪来源的干细胞内在表达高水平的多能性因子，如碱性成纤维细胞生长因子（bFGF）、转化生长因子-β（TGF-β）、纤连蛋白和 vitronectin，并且可以作为自体和异体细胞的饲养层。这些结果表明脂肪来源的细胞在再生治疗中有很大的潜力，并且可以作为研究无饲养层 iPS 生成和维持的分子机制的模型。
