U.S. Army Medical Research Institute of Infectious Diseases, 1301 Ditto Avenue, Fort Detrick, MD 21702, USA.
Am J Trop Med Hyg. 2010 Feb;82(2):318-23. doi: 10.4269/ajtmh.2010.09-0361.
Monkeypox virus (MPXV), a member of the family Poxviridae and genus Orthopoxvirus, causes a smallpox-like disease in humans. A previously described pan-Orthopoxvirus assay, based on a broad-range polymerase chain reaction (PCR) coupled with electrospray ionization mass spectrometry (PCR/ESI-MS), was evaluated for its ability to detect MPXV from spiked human and aerosol-infected cynomolgous macaque (Macaca fascicularis) samples. Detection of MPXV DNA from macaque tissue, blood, and spiked human blood by the PCR/ESI-MS pan-Orthopoxvirus assay was comparable, albeit at slightly higher levels, to the current gold standard method of real-time PCR with the pan-Orthopoxvirus assay and had a limit of detection of 200 plaque-forming units. Furthermore, the platform was able to distinguish MPXV and vaccinia viruses that were spiked into macaque blood samples at various concentrations. This platform provides a new tool for the diagnosis and monitoring of orthopoxviral loads during vaccine or antiviral studies, but also could provide rapid identification during natural outbreaks or bioterrorism attacks.
猴痘病毒（MPXV）是痘病毒科和正痘病毒属的一员，可导致人类出现类似天花的疾病。此前描述的一种基于广谱聚合酶链反应（PCR）结合电喷雾电离质谱（PCR/ESI-MS）的全正痘病毒检测方法，用于检测经人工接种和雾化感染恒河猴（Macaca fascicularis）样本中的 MPXV。PCR/ESI-MS 全正痘病毒检测方法可检测猴组织、血液和人工接种的人血中的 MPXV DNA，其检测结果与实时 PCR 全正痘病毒检测方法相当（尽管略高），检测限为 200 噬菌斑形成单位。此外，该平台还能够区分接种到不同浓度恒河猴血液样本中的猴痘病毒和牛痘病毒。该平台为疫苗或抗病毒研究期间的正痘病毒载量的诊断和监测提供了一种新工具，但也可以在自然暴发或生物恐怖袭击期间提供快速鉴定。
