Department of Surgery, Tufts Medical Center, Boston, Massachusetts, USA.
Nat Protoc. 2010 Feb;5(2):335-41. doi: 10.1038/nprot.2009.243. Epub 2010 Feb 4.
Mechanistic studies of acute pancreatitis require animal models because clinical material is generally not available during the early phases of the disease. Here we describe a protocol to induce biliary pancreatitis by retrogradely infusing bile acids into the pancreatic duct of anesthetized mice. The resulting model replicates events believed to be responsible for the onset of clinical biliary (i.e., gallstone) pancreatitis and creates highly reproducible pancreatitis with a severity that depends on the concentration of infused bile acid. Pancreatitis reaches its maximal level of severity within 24 h of induction, and it resolves over the subsequent week. This protocol enables the investigator to use genetically modified strains of mice, and it requires only relatively simple and easily learned techniques of small animal surgery. With practice and gentle technique, the surgery (from induction of anesthesia to completion of the infusion) can be completed within 25 min per animal.
机制研究的急性胰腺炎需要动物模型，因为临床资料通常是不可用的在疾病的早期阶段。在这里，我们描述了一个方案，通过逆行输注胆汁酸到麻醉小鼠的胰管来诱导胆源性胰腺炎。该模型复制了被认为是导致临床胆源性（即胆石症）胰腺炎发病的事件，并产生了高度可重现的胰腺炎，其严重程度取决于输注胆汁酸的浓度。胰腺炎在诱导后 24 小时内达到最严重程度，并在随后的一周内缓解。该方案使研究人员能够使用基因修饰的小鼠品系，并且只需要相对简单和容易学习的小动物手术技术。通过实践和熟练的技术，手术（从麻醉诱导到输注完成）可以在每只动物 25 分钟内完成。
