Department of Conservative Dentistry, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramoto-cho, Tokushima, Tokushima, 770-8504, Japan.
Department of Oral Health Care Promotion, Institute of Biomedical Sciences, Tokushima University Graduate School, Tokushima, Tokushima, Japan.
Inflammation. 2017 Apr;40(2):360-365. doi: 10.1007/s10753-016-0482-4.
Gomisin N, which is a lignan isolated from Schisandra chinensis, has some pharmacological effects. However, the anti-inflammatory effects of gomisin N on periodontal disease are uncertain. The aim of this study was to examine the effect of gomisin N on inflammatory mediator production in tumor necrosis factor (TNF)-α-stimulated human periodontal ligament cells (HPDLC). Gomisin N inhibited interleukin (IL)-6, IL-8, CC chemokine ligand (CCL) 2, and CCL20 production in TNF-α-stimulated HPDLC in a dose-dependent manner. Moreover, we revealed that gomisin N could suppress extracellular signal-regulated kinase (ERK) and c-Jun N terminal kinase (JNK) phosphorylation in TNF-α-stimulated HPDLC though protein kinase B (Akt) phosphorylation was not suppressed by gomisin N treatment. In summary, gomisin N might exert anti-inflammatory effects by attenuating cytokine production in periodontal ligament cells via inhibiting the TNF-α-stimulated ERK and JNK pathways activation.
五味子素 N 是从五味子中分离得到的木脂素，具有多种药理作用。然而，关于五味子素 N 对牙周病的抗炎作用尚不清楚。本研究旨在探讨五味子素 N 对肿瘤坏死因子 (TNF)-α 刺激的人牙周韧带细胞 (HPDLC) 中炎症介质产生的影响。五味子素 N 呈剂量依赖性抑制 TNF-α 刺激的 HPDLC 中白细胞介素 (IL)-6、IL-8、CC 趋化因子配体 (CCL) 2 和 CCL20 的产生。此外，我们发现五味子素 N 可以抑制 TNF-α 刺激的 HPDLC 中细胞外信号调节激酶 (ERK) 和 c-Jun N 末端激酶 (JNK) 的磷酸化，而蛋白激酶 B (Akt) 的磷酸化不受五味子素 N 处理的抑制。综上所述，五味子素 N 可能通过抑制 TNF-α 刺激的 ERK 和 JNK 通路的激活，减轻牙周韧带细胞中细胞因子的产生，从而发挥抗炎作用。
