Tian Yu, Xu Li, Meng Huan Xin, Ren Xiu Yun, Chen Zhi Bin, Zhang Li, Liu Kai Ning
Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing 100081, China.
Beijing Da Xue Xue Bao Yi Xue Ban. 2010 Feb 18;42(1):28-32.
To analyze the correlation between polymorphisms of the vitamin D receptor gene (Taq I and Fok I) and the aggressive periodontitis by FBAT method.
93 AgP nuclear families including 93 probands and their 155 relatives were recruited. The genotype frequency and polymorphism for VDR for the patients and their pedigree were detected by using polymerase chain reaction (PCR) combined with restriction fragment length polymorphism (RFLP).
The frequency of allele T and t accounted for 94.6 % and 5.4 % in the total populations. No tt genotype were detected. The fathers of probands carried more allele t than the mothers(9.8% vs 1.6%, P=0.005). The frequency of allele F and f accounted for 57.1 % and 42.9 % in the total populations. The result of family based associated test (FBAT) including additive model, dominant model and recessive model showed that different alleles of Taq I and Fok I had no correlation with the onset of AgP(P>0.05).
This was the first time to detect the VDR gene polymorphisms in Chinese families. The result of FBAT analysis can not show the correlation between vitamin D receptor gene polymorphisms (Taq I and Fok I) and the onset of AgP.
采用FBAT方法分析维生素D受体基因（Taq I和Fok I）多态性与侵袭性牙周炎之间的相关性。
招募93个侵袭性牙周炎核心家庭，包括93名先证者及其155名亲属。采用聚合酶链反应（PCR）结合限制性片段长度多态性（RFLP）检测患者及其家系中VDR的基因型频率和多态性。
等位基因T和t在总人群中的频率分别为94.6%和5.4%。未检测到tt基因型。先证者的父亲携带的等位基因t比母亲多（9.8%对1.6%，P = 0.005）。等位基因F和f在总人群中的频率分别为57.1%和42.9%。基于家系的关联检验（FBAT）结果，包括加性模型、显性模型和隐性模型，显示Taq I和Fok I的不同等位基因与侵袭性牙周炎的发病无相关性（P>0.05）。
这是首次在中国家庭中检测VDR基因多态性。FBAT分析结果未显示维生素D受体基因多态性（Taq I和Fok I）与侵袭性牙周炎发病之间的相关性。
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