Sun J L, Meng H X, Cao C F, Tachi Y, Shinohara M, Ueda M, Imai H, Ohura K
Department of Periodontology, Peking University School of Stomatology, 22 Zhong Guan Cun Nan Da Jie, Haidian District, 100081, Beijing, P. R. China.
J Periodontal Res. 2002 Aug;37(4):263-7. doi: 10.1034/j.1600-0765.2002.01605.x.
Recent studies have shown that vitamin D receptor (VDR) gene polymorphism had regulatory effects on bone mineral density (BMD) and bone turnover. The VDR gene has also been indicated as a candidate gene for the susceptibility of osteoporosis. However, it is unclear whether VDR genotypes could be associated with alveolar bone loss of patients with periodontitis, or whether vitamin D receptor gene could be a candidate gene for susceptibility to periodontitis. The purpose of this study was to answer these two questions.
Twenty-four cases of adult periodontitis (AP), 37 cases of early onset periodontitis (EOP) and 39 healthy controls were recruited for the study. Individual samples of venous blood and DNA were obtained from each subject. Genotypes of the TaqI VDR gene were determined by PCR and TaqI restriction endonuclease digestion.
One out of 24 AP patients, nine out of 37 EOP patients and two out of 39 healthy controls were detected with Tt genotype, while the rest had the TT genotype. The detected frequency of Tt genotype was significantly higher in EOP patients (24.3%) than in AP patients (4.2%) and healthy controls (5.1%). The frequency of t allele was also significantly higher in EOP patients. There was no statistical difference in the distribution of TaqI VDR genotypes between AP patients and healthy controls. The study suggests that Tt genotype might be a risk indicator for the susceptibility to EOP. Carriage of the allele (t) of the TaqI VDR gene may increase the risk of developing EOP.
近期研究表明，维生素D受体（VDR）基因多态性对骨密度（BMD）和骨转换具有调节作用。VDR基因也被认为是骨质疏松易感性的候选基因。然而，VDR基因型是否与牙周炎患者的牙槽骨丧失有关，或者维生素D受体基因是否可能是牙周炎易感性的候选基因尚不清楚。本研究的目的是回答这两个问题。
招募24例成人牙周炎（AP）患者、37例早发性牙周炎（EOP）患者和39例健康对照者进行研究。从每个受试者获取静脉血和DNA的个体样本。通过PCR和TaqI限制性内切酶消化确定TaqI VDR基因的基因型。
24例AP患者中有1例、37例EOP患者中有9例和39例健康对照者中有2例检测到Tt基因型，其余为TT基因型。EOP患者中检测到的Tt基因型频率（24.3%）显著高于AP患者（4.2%）和健康对照者（5.1%）。EOP患者中t等位基因的频率也显著更高。AP患者和健康对照者之间TaqI VDR基因型的分布没有统计学差异。该研究表明，Tt基因型可能是EOP易感性的风险指标。TaqI VDR基因的等位基因（t）携带可能增加患EOP的风险。
