采用安培检测微芯片电泳法分离和检测过氧亚硝酸盐。
Separation and detection of peroxynitrite using microchip electrophoresis with amperometric detection.
出版信息
Anal Chem. 2010 Mar 1;82(5):1608-11. doi: 10.1021/ac902821v.
Abstract
Peroxynitrite (ONOO(-)) is a highly reactive species implicated in the pathology of several cardiovascular and neurodegenerative diseases. It is generated in vivo by the diffusion-limited reaction of nitric oxide (NO()) and superoxide anion (()O(2)(-)) and is known to be produced during periods of inflammation. Detection of ONOO(-) is made difficult by its short half-life under physiological conditions (approximately 1 s). Here we report a method for the separation and detection of ONOO(-) from other electroactive species utilizing a microchip electrophoresis device incorporating an amperometric detection scheme. Microchip electrophoresis permits shorter separation times (approximately 25 s for ONOO(-)) and higher temporal resolution than conventional capillary electrophoresis (several minutes). This faster analysis allows ONOO(-) to be detected before substantial degradation occurs, and the increased temporal resolution permits more accurate tracking of dynamic changes in chemical systems.
摘要
过氧亚硝酸盐（ONOO(-)）是一种高度反应性的物质，与几种心血管和神经退行性疾病的病理学有关。它是由一氧化氮（NO())和超氧阴离子(()O(2)(-))的扩散限制反应在体内产生的，已知在炎症期间会产生。由于其在生理条件下的半衰期短（约 1 秒），因此很难检测到 ONOO(-)。在这里，我们报告了一种利用微芯片电泳装置分离和检测 ONOO(-)的方法，该装置结合了电流检测方案。微芯片电泳比传统的毛细管电泳（几分钟）具有更短的分离时间（ONOO(-)约为 25 秒）和更高的时间分辨率。这种更快的分析可以在 ONOO(-)发生大量降解之前进行检测，并且增加的时间分辨率可以更准确地跟踪化学系统中的动态变化。
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