细胞色素 c 氧化酶 IV mRNA 轴突运输的调控。
Regulation of axonal trafficking of cytochrome c oxidase IV mRNA.
机构信息
Laboratory of Molecular Biology, National Institute of Mental Health, National Institutes of Health, Bethesda, MD 20892-1381, USA.
出版信息
Mol Cell Neurosci. 2010 Apr;43(4):422-30. doi: 10.1016/j.mcn.2010.01.009. Epub 2010 Feb 6.
Abstract
Trafficking and local translation of axonal mRNAs play a critical role in the development and function of this neuronal subcellular structural domain. In this report, we studied cytochrome c oxidase subunit IV (COXIV) mRNA trafficking into distal axons of primary superior cervical ganglia (SCG) neurons, and provided evidence that axonal trafficking and mitochondrial association of the mRNA are mediated by an element located in a 38bp-long, hairpin-loop forming region within the 3'UTR of the transcript. Our results also suggest that suppression of local translation of COXIV mRNA results in significant attenuation of axonal elongation. Taken together, the results provide the first evidence for the existence of a cis-acting axonal transport element within a nuclear-encoding mitochondrial gene, and demonstrate the importance of the axonal trafficking and local translation of nuclear-encoded mitochondrial mRNAs in axonal growth.
摘要
轴突 mRNA 的贩运和局部翻译在这个神经元亚细胞结构域的发育和功能中起着关键作用。在本报告中，我们研究了细胞色素 c 氧化酶亚基 IV（COXIV）mRNA 向初级颈上交感神经节（SCG）神经元的远端轴突的贩运，并提供了证据表明，mRNA 的轴突贩运和线粒体缔合是由位于转录本 3'UTR 内的 38bp 长发夹环形成区域中的一个元件介导的。我们的结果还表明，抑制 COXIV mRNA 的局部翻译会导致轴突伸长显著减弱。总之，这些结果首次提供了核编码线粒体基因内存在顺式作用的轴突运输元件的证据，并证明了核编码线粒体 mRNA 的轴突运输和局部翻译在轴突生长中的重要性。
相似文献
1
Regulation of axonal trafficking of cytochrome c oxidase IV mRNA.细胞色素 c 氧化酶 IV mRNA 轴突运输的调控。
Mol Cell Neurosci. 2010 Apr;43(4):422-30. doi: 10.1016/j.mcn.2010.01.009. Epub 2010 Feb 6.
2
Molecular determinants of cytochrome C oxidase IV mRNA axonal trafficking.细胞色素C氧化酶IV信使核糖核酸轴突运输的分子决定因素。
Mol Cell Neurosci. 2017 Apr;80:32-43. doi: 10.1016/j.mcn.2017.01.008. Epub 2017 Feb 1.
3
Dysregulation of the axonal trafficking of nuclear-encoded mitochondrial mRNA alters neuronal mitochondrial activity and mouse behavior.核编码的线粒体mRNA轴突运输失调会改变神经元线粒体活性和小鼠行为。
Dev Neurobiol. 2014 Mar;74(3):333-50. doi: 10.1002/dneu.22141. Epub 2013 Nov 20.
4
Disruption of the Axonal Trafficking of Tyrosine Hydroxylase mRNA Impairs Catecholamine Biosynthesis in the Axons of Sympathetic Neurons.酪氨酸羟化酶 mRNA 的轴突运输中断会损害交感神经元轴突中的儿茶酚胺生物合成。
eNeuro. 2017 Jun 16;4(3). doi: 10.1523/ENEURO.0385-16.2017. eCollection 2017 May-Jun.
5
MicroRNA-338 regulates the axonal expression of multiple nuclear-encoded mitochondrial mRNAs encoding subunits of the oxidative phosphorylation machinery.微小RNA-338调节多种核编码线粒体信使核糖核酸的轴突表达，这些信使核糖核酸编码氧化磷酸化机制的亚基。
Cell Mol Life Sci. 2012 Dec;69(23):4017-27. doi: 10.1007/s00018-012-1064-8. Epub 2012 Jul 8.
6
Angiotensin II mediates the axonal trafficking of tyrosine hydroxylase and dopamine β-hydroxylase mRNAs and enhances norepinephrine synthesis in primary sympathetic neurons.血管紧张素 II 介导线粒体酪氨酸羟化酶和多巴胺β-羟化酶 mRNA 的轴突运输，并增强原代交感神经元中去甲肾上腺素的合成。
J Neurochem. 2019 Sep;150(6):666-677. doi: 10.1111/jnc.14821. Epub 2019 Jul 31.
7
MicroRNA-338 regulates local cytochrome c oxidase IV mRNA levels and oxidative phosphorylation in the axons of sympathetic neurons.微小RNA-338调节交感神经元轴突中局部细胞色素c氧化酶IV信使核糖核酸水平及氧化磷酸化。
J Neurosci. 2008 Nov 19;28(47):12581-90. doi: 10.1523/JNEUROSCI.3338-08.2008.
8
A heterogeneous population of nuclear-encoded mitochondrial mRNAs is present in the axons of primary sympathetic neurons.在初级交感神经元的轴突中存在着核编码线粒体mRNA的异质群体。
Mitochondrion. 2016 Sep;30:18-23. doi: 10.1016/j.mito.2016.06.002. Epub 2016 Jun 15.
9
Local translation of ATP synthase subunit 9 mRNA alters ATP levels and the production of ROS in the axon.ATP 合酶亚基 9 mRNA 的局部翻译改变了轴突中的 ATP 水平和 ROS 的产生。
Mol Cell Neurosci. 2012 Mar;49(3):263-70. doi: 10.1016/j.mcn.2011.12.006. Epub 2011 Dec 21.
10
Axonal localization and mitochondrial association of precursor microRNA 338.前体微小RNA 338的轴突定位与线粒体关联
Cell Mol Life Sci. 2016 Nov;73(22):4327-4340. doi: 10.1007/s00018-016-2270-6. Epub 2016 May 26.
引用本文的文献
1
Regulation of Subcellular Protein Synthesis for Restoring Neural Connectivity.用于恢复神经连接的亚细胞蛋白质合成调控
Int J Mol Sci. 2025 Jul 28;26(15):7283. doi: 10.3390/ijms26157283.
2
Metabolic Regulation of Mitochondrial Protein Biogenesis from a Neuronal Perspective.从神经细胞角度看线粒体蛋白生物发生的代谢调控
Biomolecules. 2022 Oct 29;12(11):1595. doi: 10.3390/biom12111595.
3
Co-transport of the nuclear-encoded Cox7c mRNA with mitochondria along axons occurs through a coding-region-dependent mechanism.核编码的 Cox7c mRNA 与线粒体通过一种依赖编码区的机制共运输到轴突中。
J Cell Sci. 2022 Aug 15;135(16). doi: 10.1242/jcs.259436. Epub 2022 Aug 16.
4
Heroin Addiction Induces Axonal Transport Dysfunction in the Brain Detected by In Vivo MRI.海洛因成瘾通过体内 MRI 检测到大脑中的轴突运输功能障碍。
Neurotox Res. 2022 Aug;40(4):1070-1085. doi: 10.1007/s12640-022-00533-3. Epub 2022 Jun 27.
5
mtIF3 is locally translated in axons and regulates mitochondrial translation for axonal growth.mtIF3 在轴突中局部翻译，并调节线粒体翻译以促进轴突生长。
BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1186/s12915-021-01215-w.
6
The functional organization of axonal mRNA transport and translation.轴突 mRNA 运输和翻译的功能组织。
Nat Rev Neurosci. 2021 Feb;22(2):77-91. doi: 10.1038/s41583-020-00407-7. Epub 2020 Dec 7.
7
Molecular mechanisms behind mRNA localization in axons.mRNA 在轴突中定位的分子机制。
Open Biol. 2020 Sep;10(9):200177. doi: 10.1098/rsob.200177. Epub 2020 Sep 23.
8
Object-Based Analyses in FIJI/ImageJ to Measure Local RNA Translation Sites in Neurites in Response to Aβ1-42 Oligomers.使用FIJI/ImageJ基于对象的分析方法来测量神经突中响应Aβ1-42寡聚体的局部RNA翻译位点。
Front Neurosci. 2020 Jun 3;14:547. doi: 10.3389/fnins.2020.00547. eCollection 2020.
9
Altered Mitochondrial Dynamics in Motor Neuron Disease: An Emerging Perspective.运动神经元病中线粒体动力学的改变：一个新视角
Cells. 2020 Apr 24;9(4):1065. doi: 10.3390/cells9041065.
10
Mechanisms Linking Mitochondrial Dysfunction and Proteostasis Failure.线粒体功能障碍与蛋白质稳态失调的关联机制。
Trends Cell Biol. 2020 Apr;30(4):317-328. doi: 10.1016/j.tcb.2020.01.008. Epub 2020 Feb 12.
本文引用的文献
1
Axonal mRNA in uninjured and regenerating cortical mammalian axons.未损伤和再生的哺乳动物皮质轴突中的轴突mRNA
J Neurosci. 2009 Apr 15;29(15):4697-707. doi: 10.1523/JNEUROSCI.6130-08.2009.
2
Axonal protein synthesis and the regulation of local mitochondrial function.轴突蛋白合成与局部线粒体功能的调节
Results Probl Cell Differ. 2009;48:225-42. doi: 10.1007/400_2009_1.
3
Mitochondria in neuroplasticity and neurological disorders.线粒体在神经可塑性和神经疾病中的作用
Neuron. 2008 Dec 10;60(5):748-66. doi: 10.1016/j.neuron.2008.10.010.
4
MicroRNA-338 regulates local cytochrome c oxidase IV mRNA levels and oxidative phosphorylation in the axons of sympathetic neurons.微小RNA-338调节交感神经元轴突中局部细胞色素c氧化酶IV信使核糖核酸水平及氧化磷酸化。
J Neurosci. 2008 Nov 19;28(47):12581-90. doi: 10.1523/JNEUROSCI.3338-08.2008.
5
Mitochondrial biogenesis in the axons of vertebrate peripheral neurons.脊椎动物外周神经元轴突中的线粒体生物合成。
Dev Neurobiol. 2008 Sep 15;68(11):1348-61. doi: 10.1002/dneu.20668.
6
Mitochondrial involvement in psychiatric disorders.线粒体与精神疾病的关联。
Ann Med. 2008;40(4):281-95. doi: 10.1080/07853890801923753.
7
Structures, kinetics, thermodynamics, and biological functions of RNA hairpins.RNA发夹结构、动力学、热力学及生物学功能
Annu Rev Phys Chem. 2008;59:79-103. doi: 10.1146/annurev.physchem.59.032607.093743.
8
Extracellular stimuli specifically regulate localized levels of individual neuronal mRNAs.细胞外刺激特异性地调节单个神经元mRNA的局部水平。
J Cell Biol. 2007 Sep 10;178(6):965-80. doi: 10.1083/jcb.200703209. Epub 2007 Sep 4.
9
Axon viability and mitochondrial function are dependent on local protein synthesis in sympathetic neurons.轴突的活力和线粒体功能依赖于交感神经元中的局部蛋白质合成。
Cell Mol Neurobiol. 2007 Sep;27(6):701-16. doi: 10.1007/s10571-007-9148-y. Epub 2007 Jul 6.
10
Cis-acting determinants of asymmetric, cytoplasmic RNA transport.不对称细胞质RNA转运的顺式作用决定因素。
RNA. 2007 May;13(5):625-42. doi: 10.1261/rna.262607.
Zotero 插件