INRS-Institut Armand-Frappier, Université du Québec, 531 Boulevard des Prairies, Laval, Québec H7V 1B7, Canada.
J Virol. 2010 May;84(9):4810-5. doi: 10.1128/JVI.02437-09. Epub 2010 Feb 10.
Herpes simplex virus 1 (HSV-1) induces relocalization of several nucleolar proteins. We have found that, as for fibrillarin, the HSV-1-induced redistribution of two RNA polymerase I components, upstream binding factor (UBF) and RPA194, was independent of the viral protein UL24, which affects nucleolin localization. Nevertheless, the kinetics and sites of redistribution for fibrillarin and UBF differed. Interestingly, UBF remained associated with RPA194 during infection. Although UBF is redistributed to viral replication compartments during infection, we did not detect foci of UBF at early sites of viral DNA synthesis, suggesting that it may not be directly involved in this process at early times.
单纯疱疹病毒 1（HSV-1）诱导几种核仁蛋白的重定位。我们发现，与核仁蛋白 f ibrillarin 一样，HSV-1 诱导的 RNA 聚合酶 I 两个成分，上游结合因子（UBF）和 RPA194 的重新分布，不依赖于影响核仁蛋白定位的病毒蛋白 UL24。然而，核仁蛋白 f ibrillarin 和 UBF 的重分布动力学和位置不同。有趣的是，UBF 在感染过程中仍与 RPA194 相关联。虽然 UBF 在感染过程中被重新分配到病毒复制区，但我们没有在病毒 DNA 合成的早期部位检测到 UBF 焦点，这表明它在早期可能没有直接参与这一过程。
