School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia.
FEBS J. 2010 Apr;277(7):1606-17. doi: 10.1111/j.1742-4658.2010.07587.x. Epub 2010 Feb 10.
A higher prevalence of Campylobacter concisus and higher levels of IgG antibodies specific to C. concisus in Crohn's disease patients than in controls were recently detected. In this study, 1D and 2D gel electrophoresis coupled with LTQ FT-MS and QStar tandem MS, respectively, were performed to characterize the secretome of a C. concisus strain isolated from a Crohn's disease patient. Two hundred and one secreted proteins were identified, of which 86 were bioinformatically predicted to be secreted. Searches were performed on the genome of C. concisus strain 13826, and 25 genes that have been associated with virulence or colonization in other organisms were identified. The zonula occludens toxin was found only in C. concisus among the Campylobacterales, although expanded searches revealed that this protein was present in two epsilon-proteobacterial species from extreme marine environments. Alignments and structural threading indicated that this toxin shared features with that of other virulent pathogens, including Neisseria meningitidis and Vibrio cholerae. Further comparative analyses identified several associations between the secretome of C. consisus and putative virulence factors of this bacterium. This study has identified several factors putatively associated with disease outcome, suggesting that C. concisus is a pathogen of the gastrointestinal tract.
最近发现，与对照组相比，克罗恩病患者中弯曲菌属的患病率更高，且针对弯曲菌属的 IgG 抗体水平也更高。在这项研究中，分别采用 1D 和 2D 凝胶电泳结合 LTQ FT-MS 和 QStar 串联 MS 技术，对从克罗恩病患者中分离出的弯曲菌属菌株的分泌组进行了特征分析。鉴定出了 201 种分泌蛋白，其中 86 种经生物信息学预测为分泌蛋白。在弯曲菌属 13826 株的基因组上进行了搜索，鉴定出了与其他生物体中的毒力或定植相关的 25 个基因。在弯曲菌目中，仅在弯曲菌属中发现了紧密连接毒素，尽管扩大搜索发现，这种蛋白存在于两种来自极端海洋环境的 ε-变形菌中。比对和结构穿线表明，这种毒素与其他毒力病原体（包括脑膜炎奈瑟菌和霍乱弧菌）的毒素具有相似特征。进一步的比较分析确定了弯曲菌属的分泌组与该细菌的一些假定毒力因子之间的几种关联。本研究鉴定出了几种与疾病结果相关的假定因素，表明弯曲菌属是胃肠道病原体。
