Clinic of Anesthesiology/Critical Care Medicine and Pain Therapy (M.A.B., P.C), Ludwig-Maximilians-University, City of Munich, Germany.
Eur J Med Res. 2009;14(12):526-31. doi: 10.1186/2047-783x-14-12-526.
Increased vascular permeability is a characteristic feature of sepsis which, in the past, has been ascribed exclusively to a malfunction of endothelial cells. However, recently it has become evident that the endothelial glycocalyx is of considerable importance concerning various aspects of vascular physiology, e.g. the vascular barrier and inflammation. Heparan sulfate, one of its essential components is characteristically traceable in blood, in case the endothelial glycocalyx is damaged or destroyed.
In 15 pigs we investigated whether the administration of endotoxin from gram-negative bacteria (Escherichia coli) results in increased serum levels of heparan sulfate, signalizing a shedding of the glycocalyx. In addition, markers of inflammation (white blood cell count, platelet count, tumour necrosis factor-α and interleukin-6) were evaluated over an observation period of 6 hours.
Serum heparan sulfate concentrations significantly increased over time in the endotoxin group and were significantly elevated in comparison to the control group 6 hours after administration of endotoxin (p<0.001). In the endotoxin group all markers of inflammation significantly changed during the time course.
The administration of bacterial endotoxin induced a significant rise in degradation products of the endothelial glycocalyx.
血管通透性增加是败血症的一个特征，过去，这被归因于内皮细胞的功能障碍。然而，最近人们已经清楚地认识到，内皮糖萼对于血管生理学的各个方面都具有相当重要的意义，例如血管屏障和炎症。其重要组成部分之一的肝素硫酸盐，在内皮糖萼受损或破坏的情况下，会在血液中被检测到。
在 15 头猪中，我们研究了革兰氏阴性菌（大肠杆菌）内毒素的给药是否会导致肝素硫酸盐血清水平升高，提示糖萼脱落。此外，还评估了炎症标志物（白细胞计数、血小板计数、肿瘤坏死因子-α和白细胞介素-6）在 6 小时的观察期内的变化。
内毒素组的血清肝素硫酸盐浓度随时间显著增加，并且在给药后 6 小时与对照组相比显著升高（p<0.001）。在内毒素组中，所有炎症标志物在整个时间过程中均显著变化。
细菌内毒素的给药诱导了内皮糖萼降解产物的显著增加。
