Département de Dermatologie, CHRU Dupuytren, 2, Avenue Martin Luther King, 87042 Limoges, France.
Anticancer Res. 2010 Jan;30(1):143-7.
The large number of studies devoted to the effect of ultraviolet light on biological systems, contrasts with the lack of experimental data concerning the direct effects of visible light. It has been shown that blue light inhibited the growth of B16F10 melanoma cell lines and reduced the percentage of S phase cells. Yet these effects are poorly understood.
Two cell lines and irradiation with blue light were used. Cell mortality and a possible mechanism of action were investigated.
Exposure of B16F10 melanoma and bovine endothelial cells to blue light (wavelength 450 nm, 10 J/cm(2) from a Waldman lamp) induced a rapid and large reduction in viability followed by the death of virtually all the irradiated cells within 24 h. These results led us to expose a patient with haemorrhagic cutaneous melanoma metastasis to blue light. Irradiation led to an immediate arrest of haemorrhage, an inhibition of tumour growth and extensive tumour necrosis 24h after irradiation.
Exposure to blue light may offer new approaches to the treatment of superficial skin carcinomas in humans.
大量研究致力于研究紫外线对生物系统的影响，而缺乏有关可见光直接影响的实验数据。已经表明，蓝光抑制 B16F10 黑色素瘤细胞系的生长，并降低 S 期细胞的百分比。然而，这些影响还不太清楚。
使用了两种细胞系和蓝光照射。研究了细胞死亡率和可能的作用机制。
B16F10 黑色素瘤和牛内皮细胞暴露于蓝光（波长 450nm，来自 Waldman 灯的 10J/cm²）会导致细胞活力迅速而大幅下降，随后在 24 小时内几乎所有照射的细胞都会死亡。这些结果促使我们对患有出血性皮肤黑色素瘤转移的患者进行蓝光照射。照射后立即止血，肿瘤生长受到抑制，照射后 24 小时广泛肿瘤坏死。
暴露于蓝光可能为人类治疗浅表皮肤癌提供新的方法。
