Wu Xiaobin, Park Jongho, Chow Siu Yu A, Kasuya Maria Carmelita Z, Ikeuchi Yoshiho, Kim Beomjoon
Institute of Industrial Science, The University of Tokyo, Tokyo, Japan.
Department of Precision Engineering, School of Engineering, The University of Tokyo, Japan.
Biomed Opt Express. 2022 Jan 31;13(2):1045-1060. doi: 10.1364/BOE.450456. eCollection 2022 Feb 1.
Light-based therapy is an emerging treatment for skin cancer, which has received increased attention due to its drug-free and non-invasive approach. However, the limitation of current light therapy methods is the inability for light to penetrate the skin and reach deep lesions. As such, we have developed a polylactic acid (PLA) microneedles array as a novel light transmission platform to perform evaluation regarding the effect of light therapy on skin cancer. For the first time, we designed and fabricated a microneedle array system with a height fixation device that can be installed in a cell culture dish and an LED array for blue light irradiation. The effect of the blue light combined with the microneedles on cell apoptosis was evaluated using B16F10 melanoma cells and analyzed by Hoechst staining. Our results demonstrate that blue light can be transmitted by microneedles to skin cells and effectively affect cell viability.
光基疗法是一种新兴的皮肤癌治疗方法，因其无药物和非侵入性的治疗方式而受到越来越多的关注。然而，当前光疗方法的局限性在于光线无法穿透皮肤并到达深层病变部位。因此，我们开发了一种聚乳酸（PLA）微针阵列作为新型光传输平台，以评估光疗对皮肤癌的治疗效果。我们首次设计并制造了一种带有高度固定装置的微针阵列系统，该装置可安装在细胞培养皿中，并配备用于蓝光照射的LED阵列。使用B16F10黑色素瘤细胞评估蓝光与微针联合作用对细胞凋亡的影响，并通过Hoechst染色进行分析。我们的结果表明，蓝光可通过微针传输至皮肤细胞并有效影响细胞活力。
