Chen Zeqing, Li Wenqi, Hu Xiaojian, Liu Muqing
Academy for Engineering and Technology, Fudan University, 220th Handan Road, Shanghai,200433, China.
Institute for Electric Light Sources, Fudan University, 220th Handan Road, Shanghai, 200433, China.
Biomed Opt Express. 2019 Dec 4;11(1):27-39. doi: 10.1364/BOE.11.000027. eCollection 2020 Jan 1.
Melanoma is a type of aggressive cancer. Recent studies have indicated that blue light has an inhibition effect on melanoma cells, but the effect of photobiomodulation (PBM) parameters on the treatment of melanoma remains unknown. Thus, this study was aimed to investigate B16F10 melanoma cells responses to PBM with varying irradiance and doses, and further explored the molecular mechanism of PBM. Our results suggested that the responses of B16F10 melanoma cells to PBM with varying irradiance and dose were different and the inhibition of blue light on cells under high irradiance was better than low irradiance at a constant total dose (0.04, 0.07, 0.15, 0.22, 0.30, 0.37, 0.45, 0.56 or 1.12 J/cm), presumably due to that high irradiance can produce more ROS, thus disrupting mitochondrial function.
黑色素瘤是一种侵袭性癌症。最近的研究表明，蓝光对黑色素瘤细胞具有抑制作用，但光生物调节（PBM）参数对黑色素瘤治疗的影响尚不清楚。因此，本研究旨在研究B16F10黑色素瘤细胞对不同辐照度和剂量的PBM的反应，并进一步探索PBM的分子机制。我们的结果表明，B16F10黑色素瘤细胞对不同辐照度和剂量的PBM的反应不同，在总剂量恒定（0.04、0.07、0.15、0.22、0.30、0.37、0.45、0.56或1.12 J/cm）时，蓝光在高辐照度下对细胞的抑制作用优于低辐照度，这可能是因为高辐照度可产生更多的活性氧，从而破坏线粒体功能。
