Department of Periodontology, Peking University School and Hospital of Stomatology, Beijing, China.
J Periodontol. 2010 Feb;81(2):260-6. doi: 10.1902/jop.2009.090355.
25-hydroxy vitamin D(3) is the major circulating metabolite of vitamin D. Elevated plasma 25-hydroxy vitamin D(3) levels were verified to be associated with generalized aggressive periodontitis (GAgP). In the present study, the influence of initial periodontal therapy on systemic and local levels of 25-hydroxy vitamin D(3) and three related elements (osteocalcin and interleukin-1beta and -6) in patients with GAgP was investigated.
Nineteen patients with GAgP were enrolled. All patients received initial periodontal therapy. Gingival crevicular fluid at two sites of each subject were obtained before therapy and 2 and 6 months after therapy. Plasma was obtained before and 2 months after therapy from 12 of 19 subjects. Systemic and local levels of 25-hydroxy vitamin D(3), osteocalcin, and interleukin-1beta and -6 before and after therapy were measured using radioimmunoassay or enzyme-linked immunosorbent assay kits and compared.
The respective systemic 25-hydroxy vitamin D(3) and interleukin-1beta levels significantly dropped from baseline to 2 months after therapy (29.28 nmol/l versus 22.50 nmol/l, P = 0.001, and 6.71 ng/l versus 3.23 ng/l, P <0.001, respectively). The respective local 25-hydroxy vitamin D(3) and interleukin-1beta levels significantly decreased from baseline to 2 and 6 months after therapy (8,950 nmol/l versus 5,650 nmol/l versus 3,438 nmol/l, P <0.001, and 10,595 ng/l versus 5,495 ng/l versus 3,960 ng/l, P <0.001, respectively). Systemic and local 25-hydroxy vitamin D(3) concentrations were positively correlated at baseline (r = 0.877; P = 0.022), as was osteocalcin (r = 0.939; P = 0.005).
25-hydroxy vitamin D(3) and interleukin-1beta levels were systemically and locally reduced in patients with GAgP by initial periodontal therapy. 25-hydroxy vitamin D(3) might be involved in periodontal inflammation.
25-羟维生素 D（3）是维生素 D 的主要循环代谢物。已证实血浆 25-羟维生素 D（3）水平升高与广泛性侵袭性牙周炎（GAgP）有关。本研究旨在探讨初始牙周治疗对 GAgP 患者全身和局部 25-羟维生素 D（3）及三种相关元素（骨钙素、白细胞介素-1β和白细胞介素-6）水平的影响。
纳入 19 例 GAgP 患者。所有患者均接受初始牙周治疗。在治疗前和治疗后 2 个月和 6 个月，每个受试者的两个部位均获得龈沟液。19 例患者中的 12 例在治疗前和治疗后 2 个月获得血浆。采用放射免疫法或酶联免疫吸附试验试剂盒检测治疗前后系统和局部 25-羟维生素 D（3）、骨钙素和白细胞介素-1β和白细胞介素-6 水平，并进行比较。
治疗后 2 个月，系统 25-羟维生素 D（3）和白细胞介素-1β水平分别从基线时的 29.28 nmol/L 和 6.71 ng/L 显著下降至 22.50 nmol/L 和 3.23 ng/L（P=0.001 和 P<0.001）。局部 25-羟维生素 D（3）和白细胞介素-1β水平也分别从基线时的 8950 nmol/L、10595 ng/L 显著下降至治疗后 2 个月和 6 个月时的 5650 nmol/L、5495 ng/L 和 3438 nmol/L、3960 ng/L（P<0.001 和 P<0.001）。治疗前，系统和局部 25-羟维生素 D（3）浓度呈正相关（r=0.877；P=0.022），骨钙素也呈正相关（r=0.939；P=0.005）。
初始牙周治疗可降低 GAgP 患者全身和局部 25-羟维生素 D（3）和白细胞介素-1β水平。25-羟维生素 D（3）可能参与牙周炎症。
