Philipps-University Marburg, Department of Chemistry/Biochemistry, Hans-Meerwein-Strasse, Marburg, Germany.
Curr Opin Struct Biol. 2010 Apr;20(2):234-40. doi: 10.1016/j.sbi.2010.01.009. Epub 2010 Feb 10.
Nonribosomal peptide synthetases (NRPSs) are large multimodular biocatalysts that utilize complex regiospecific and stereospecific reactions to assemble structurally and functionally diverse peptides that have important medicinal applications. During this ribosome-independent peptide synthesis, catalytic domains of NRPS select, activate or modify the covalently tethered reaction intermediates to control the iterative chain elongation process and product release. Recent advances in structural elucidation of domains, didomains, and an entire termination module revealed valuable insights into the mechanism of nonribosomal synthesis and are highlighted herein.
非核糖体肽合成酶（NRPSs）是一种大型多模块生物催化剂，利用复杂的区域和立体特异性反应来组装结构和功能多样的肽，这些肽具有重要的药用应用。在这种核糖体非依赖的肽合成过程中，NRPS 的催化结构域选择、激活或修饰共价连接的反应中间体，以控制迭代链延伸过程和产物释放。最近在结构阐明领域、二结构域和整个终止模块方面的进展揭示了非核糖体合成机制的有价值的见解，本文对此进行了重点介绍。
