Laboratory of Physiology and Ethnopharmacology, UFR Physiology and Pharmacology, Department of Biology, Faculty of Sciences, University Mohamed Ist, Oujda, Morocco.
Hum Exp Toxicol. 2010 Oct;29(10):865-71. doi: 10.1177/0960327110362704. Epub 2010 Feb 12.
The goal of the present study is to test the effect of water extract (WE) of four medicinal plants used as antidiabetics in Eastern Morocco (Arbutus unedo: Au, Ammoïdes pusilla: Ap, Thymelaea hirsuta: Th, and Urtica dioïca: Ud). These plants are used in cooking to bring out the flavor in a dish or to complement it. The first experiment was realized in order to determine the antidiabetic effect of the WE of these plants during 5 weeks' treatment. Seven groups of Wistar rats were used: Healthy controls, neonatal streptozotocin (n-stz) induced-diabetic rats (90 mg/kg; intraperitoneally [i.p.]), n-stz + tolbutamide (400 mg/l), and 4 groups n-stz + WE of plants (400 mg/l, drink water). The percentages of Plasma glucose lowering effect were, respectively for Au, Ap, Th, Ud and tolbutamide: 31.6 % p<0.01, 27.4 % p<0.05, 38.2 % p<0.01, 13 % and 33.9 % p<0.05 when compared with untreated diabetic controls. In a second experiment, oral glucose tolerance tests were carried out in n-stz induced-diabetic rats. The i.p. administration of the water extract (WE) of Ap and Ud (150 mg/kg) 30 minutes before the glucose overload (2 g/kg) showed a significant reduction glycemia, respectively of 36 % at 60 min (p<0.05) and 50 % at 180 min (p<0.05) after glucose overload compared with controls. In contrast, the effect of WE of Au and Th (150 mg/kg, i.p.) was not significant. The in vitro study of glucose utilization by isolated rat hemidiaphragm suggests that these extracts in combination with insulin potentiate its activity and enhance the utilization of glucose. In conclusion, it seems that these plants possess antidiabetic activity.
本研究的目的是测试在摩洛哥东部用作抗糖尿病药物的四种药用植物的水提取物（WE）的效果（熊果酸：Au、Ammoides pusilla：Ap、Thymelaea hirsuta：Th 和荨麻：Ud）。这些植物在烹饪中用于增加菜肴的风味或补充菜肴的风味。第一个实验是为了确定这些植物的 WE 在 5 周治疗期间的抗糖尿病作用。使用了七组 Wistar 大鼠：健康对照组、新生链脲佐菌素（n-stz）诱导的糖尿病大鼠（90 mg/kg；腹腔内 [i.p.]）、n-stz + 甲苯磺丁脲（400 mg/l）和 4 组 n-stz + 植物 WE（400 mg/l，饮用水）。Au、Ap、Th、Ud 和甲苯磺丁脲分别使血浆葡萄糖降低的百分比为 31.6%p<0.01、27.4%p<0.05、38.2%p<0.01、13%和 33.9%p<0.05，与未治疗的糖尿病对照组相比。在第二个实验中，在 n-stz 诱导的糖尿病大鼠中进行了口服葡萄糖耐量试验。在葡萄糖过载（2 g/kg）前 30 分钟，腹腔内给予 Ap 和 Ud 的水提取物（WE）（150 mg/kg）可使葡萄糖过载后 60 分钟（p<0.05）和 180 分钟（p<0.05）的血糖分别显著降低 36%和 50%。相比之下，Au 和 Th 的 WE（150 mg/kg，i.p.）的作用不显著。离体大鼠膈肌葡萄糖利用的体外研究表明，这些提取物与胰岛素联合使用可增强其活性并增强葡萄糖的利用。总之，这些植物似乎具有抗糖尿病活性。
