Jacobson B E, Lockitch G, Quigley G
Department of Pathology, British Columbia Children's Hospital, Vancouver, Canada.
Clin Chem. 1991 Apr;37(4):515-9.
The effect of low concentrations of lead on pre- and post-natal growth and development is a current concern. We describe a simple method of sample preparation for direct determination of lead in whole blood by Zeeman graphite-furnace atomic absorption spectrometry. This procedure improves analytical precision and accuracy of lead determinations at low concentrations as compared with published furnace data. At blood lead concentrations of 0.25, 1.98, and 3.76 mumol/L, within-run CVs were 3.2%, 1.8%, and 1.4% respectively; between-run CVs were 7.3%, 2.9%, and 2.2%. Accuracy, as demonstrated by analytical recovery, ranged from 99% to 102%. Our reproducibility/accuracy score in the 1989 Quebec interlaboratory comparison program was 96% compared with the target, second best of 66 participating laboratories.
低浓度铅对产前和产后生长发育的影响是当前人们关注的问题。我们描述了一种简单的样品制备方法，用于通过塞曼石墨炉原子吸收光谱法直接测定全血中的铅。与已发表的炉内数据相比，该程序提高了低浓度铅测定的分析精度和准确性。在血铅浓度为0.25、1.98和3.76μmol/L时，批内变异系数分别为3.2%、1.8%和1.4%；批间变异系数分别为7.3%、2.9%和2.2%。通过分析回收率证明的准确度在99%至102%之间。在1989年魁北克实验室间比对计划中，我们的重现性/准确度得分与目标值相比为96%，在66个参与实验室中排名第二。
