DNA zyme 自组装金纳米粒子用于荧光各向异性测定法测定金属离子。
DNAzyme self-assembled gold nanoparticles for determination of metal ions using fluorescence anisotropy assay.
机构信息
Lab of Biosystems and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
出版信息
Anal Biochem. 2010 Jun 1;401(1):47-52. doi: 10.1016/j.ab.2010.02.014. Epub 2010 Feb 14.
Gold nanoparticles can be exploited to facilitate a highly sensitive and selective metal ion detection based on fluorescence anisotropy assay with metal ion-dependent DNA-cleaving DNAzyme. This assay allows rapid and accurate determination of metal ions in aqueous medium at room temperature. The method has been demonstrated for determination of Cu(2+) and Pb(2+) ions. The detection sensitivity can be significantly improved to 1 nM by using a "nanoparticle enhancement" approach. Moreover, the assay was also tested in 384-well plates for high-throughput routine determination of toxic metal ions in environmental samples. The method showed distinct advantages over conventional methods in terms of its potential sensitivity, specificity, and ability for rapid response.
金纳米粒子可用于促进基于荧光各向异性测定的高度敏感和选择性金属离子检测，该测定使用金属离子依赖性 DNA 切割 DNA 酶。该测定法允许在室温下在水介质中快速准确地测定金属离子。该方法已用于测定 Cu(2+)和 Pb(2+)离子。通过使用“纳米粒子增强”方法，检测灵敏度可显著提高至 1 nM。此外，该测定法还在 384 孔板中用于环境样品中有毒金属离子的高通量常规测定。与传统方法相比，该方法在潜在灵敏度、特异性和快速响应能力方面具有明显优势。
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