一种基于噬菌体f1基因II蛋白的新型酵母重组体。
A novel recombinator in yeast based on gene II protein from bacteriophage f1.
作者信息
Strathern J N, Weinstock K G, Higgins D R, McGill C B
机构信息
Laboratory of Eukaryotic Gene Expression, NCI-Frederick Cancer Research and Development Center, Basic Research Program, Maryland 21702-1201.
出版信息
Genetics. 1991 Jan;127(1):61-73. doi: 10.1093/genetics/127.1.61.
Abstract
Interchromosomal mitotic recombination in yeast can be stimulated by the protein encoded by gene II of bacteriophage f1. The normal role of the gene II enzyme is to make a site-specific cleavage of a particular strand of the duplex form of the bacteriophage DNA at the origin of DNA replication. The gene II protein was expressed in yeast in an attempt to determine the role of nicked DNA in the initiation of recombination. Stimulation of recombination in yeast by the gene II protein was dependent on the presence of a recognition site for gene II enzyme in the region being assayed. Recombination was stimulated in both directions from the gene II recognition site but showed a directional bias. The distribution of alleles among the recombinants indicated that the chromosome with the gene II recognition site acted as the recipient in gene conversion events.
摘要
噬菌体f1基因II编码的蛋白质可刺激酵母中的染色体间有丝分裂重组。基因II酶的正常作用是在噬菌体DNA双链形式的特定链上，于DNA复制起点处进行位点特异性切割。在酵母中表达基因II蛋白，以试图确定切口DNA在重组起始中的作用。基因II蛋白对酵母重组的刺激取决于被检测区域中基因II酶识别位点的存在。从基因II识别位点向两个方向均刺激了重组，但显示出方向偏向性。重组子中等位基因的分布表明，带有基因II识别位点的染色体在基因转换事件中作为受体。
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