Nune Satish K, Chanda Nripen, Shukla Ravi, Katti Kavita, Kulkarni Rajesh R, Thilakavathi Subramanian, Mekapothula Swapna, Kannan Raghuraman, Katti Kattesh V
Departments of Radiology, Physics, Bio-medical Sciences and Nuclear Science and Engineering Institute University of Missouri - Columbia, Columbia, MO 65212.
J Mater Chem. 2009 Jun 1;19(19):2912-2920. doi: 10.1039/b822015h.
Phytochemicals occluded in tea have been extensively used as dietary supplements and as natural pharmaceuticals in the treatment of various diseases including human cancer. Results on the reduction capabilities of phytochemicals present in tea to reduce gold salts to the corresponding gold nanoparticles are presented in this paper. The phytochemicals present in tea serve the dual roles as effective reducing agents to reduce gold and also as stabilizers to provide robust coating on the gold nanoparticles in a single step. The Tea-generated gold nanoparticles (T-AuNPs), have demonstrated remarkable in vitro stability in various buffers including saline, histidine, HSA, and cysteine solutions. T-AuNPs with phytochemical coatings have shown significant affinity toward prostate (PC-3) and breast (MCF-7) cancer cells. Results on the cellular internalization of T-AuNPs through endocytosis into the PC-3 and MCF-7 cells are presented. The generation of T-AuNPs follows all principles of green chemistry and have been found to be non toxic as assessed through MTT assays. No 'man made' chemicals, other than gold salts, are used in this true biogenic green nanotechnological process thus paving excellent opportunities for their applications in molecular imaging and therapy.
茶叶中含有的植物化学物质已被广泛用作膳食补充剂和天然药物，用于治疗包括人类癌症在内的各种疾病。本文介绍了茶叶中植物化学物质将金盐还原为相应金纳米颗粒的还原能力。茶叶中的植物化学物质具有双重作用，既作为有效的还原剂来还原金，又作为稳定剂在一步反应中为金纳米颗粒提供坚固的涂层。茶叶生成的金纳米颗粒（T-AuNPs）在包括盐水、组氨酸、人血清白蛋白和半胱氨酸溶液在内的各种缓冲液中表现出显著的体外稳定性。具有植物化学涂层的T-AuNPs对前列腺（PC-3）和乳腺（MCF-7）癌细胞表现出显著的亲和力。本文展示了T-AuNPs通过内吞作用进入PC-3和MCF-7细胞的细胞内化结果。T-AuNPs的生成遵循绿色化学的所有原则，通过MTT试验评估发现其无毒。在这个真正的生物源绿色纳米技术过程中，除了金盐之外，不使用任何“人造”化学物质，因此为它们在分子成像和治疗中的应用提供了绝佳的机会。
