Amarnath Kanchana, Mathew Nina Liza, Nellore Jayshree, Siddarth Chagam Reddy Venkat, Kumar Jayanthi
Department of Medical Biochemistry, Dental College & Hospitals, Sathyabama University, Chennai, 600119 Tamil Nadu India.
Department of Biotechnology, Sathyabama University, Chennai, 600119 Tamil Nadu India.
Cancer Nanotechnol. 2011;2(1-6):121-132. doi: 10.1007/s12645-011-0022-8. Epub 2011 Sep 15.
The remarkable health benefits of the chemical cocktails occluded within (grapes) have been broadly used as dietary supplements and as natural pharmaceuticals in the treatment of various diseases including human cancer. Current discovery demonstrates the rapid formation of gold nanoparticles with the phytochemicals present in grapes, which serve a dual role as synergistic reducing agents to reduce gold salts into gold nanoparticles and also as stabilizers to provide a robust coating on the gold nanoparticles in a single step. Furthermore, the grape-generated gold nanoparticles (GAuNPs), have demonstrated remarkable in vitro stability on specific functionalization with peptides (GSH) and thiol-containing compounds (lipoic acid) followed by the induction of cell-specific response. In addition, the grape-generated gold nanoparticles (GAuNPs, GSH-GAuNPs, LA-GAuNPs) have demonstrated remarkable affinity towards human breast cancer cells (HBL-100) in the present study. These studies thus signified the cellular internalization of GAuNPs and its conjugates by transmission electron microscopy through endocytosis into cancer cells. Notably, at higher concentration of gold nanoparticles conjugate, there was an asymmetric accumulation of gold nanoparticles in the periphery of the cell nucleus of the HBL-100 cells which was confirmed by fluorescence microscopy. Other than gold salts, no "manmade" chemicals are used in this truly biogenic, green nanotechnological process which thereby paves the way for outstanding opening for their application in molecular imaging and cancer therapy.
（葡萄）中所含化学混合物具有显著的健康益处，已被广泛用作膳食补充剂和天然药物，用于治疗包括人类癌症在内的各种疾病。目前的研究发现，葡萄中的植物化学物质能快速形成金纳米颗粒，这些植物化学物质具有双重作用，既是协同还原剂，可将金盐还原为金纳米颗粒，又是稳定剂，能一步为金纳米颗粒提供坚固的涂层。此外，葡萄生成的金纳米颗粒（GAuNPs）在用肽（GSH）和含硫醇化合物（硫辛酸）进行特定功能化处理后，表现出显著的体外稳定性，随后诱导细胞特异性反应。另外，在本研究中，葡萄生成的金纳米颗粒（GAuNPs、GSH - GAuNPs、LA - GAuNPs）对人乳腺癌细胞（HBL - 100）表现出显著的亲和力。这些研究通过透射电子显微镜表明GAuNPs及其共轭物通过内吞作用进入癌细胞的细胞内化过程。值得注意的是，在较高浓度的金纳米颗粒共轭物作用下，荧光显微镜证实HBL - 100细胞核周边存在金纳米颗粒的不对称积累。除了金盐外，在这个真正的生物源绿色纳米技术过程中未使用任何“人造”化学物质，从而为其在分子成像和癌症治疗中的应用开辟了出色的前景。
