快速鉴定及表征发菜中的抗氧化成分。

Rapid identification and characterization of antioxidants from Ligularia fischeri.

机构信息

Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing, 100081, China.

出版信息

Arch Pharm Res. 2009 Dec;32(12):1689-94. doi: 10.1007/s12272-009-2204-z. Epub 2010 Feb 17.

PMID:20162395

Abstract

The objectives of this study were to investigate the radical-scavenging activity of Ligularia fischeri on oxidative damage by the radical 1,1-diphenyl-2-picrylhydrazyl (DPPH) and to rapidly identify the active components using the bioassay-linked fractionation method. The MeOH extract and fractions of CH(2)Cl(2), BuOH, and H(2)O from L. fischeri showed DPPH radical-scavenging effects in a dose-dependent manner (p < 0.01). In particular, the BuOH fraction had the most effective (p < 0.05) antioxidative capacity. The active constituents from the BuOH fraction of L. fischeri were rapidly isolated by bioassay-linked HPLC method and identified as hyperoside and 2''-acetylhyperoside with potent antioxidant effects against the DPPH radical, with IC(50) values of 1.31 and 7.09 microg/mL, respectively. They have not been reported from L. fischeri yet.

摘要

本研究旨在探讨橐吾属植物 Ligularia fischeri 对自由基 1,1-二苯基-2-苦基肼基（DPPH）氧化损伤的清除活性，并利用生物测定相关的分段法快速鉴定其活性成分。L. fischeri 的 MeOH 提取物及 CH(2)Cl(2)、BuOH 和 H(2)O 馏分均表现出 DPPH 自由基清除活性，呈剂量依赖性（p < 0.01）。特别是 BuOH 馏分的抗氧化能力最强（p < 0.05）。通过生物测定相关 HPLC 法，从 L. fischeri 的 BuOH 馏分中快速分离出活性成分，并鉴定为具有较强清除 DPPH 自由基能力的金丝桃苷和 2''-乙酰基金丝桃苷，IC(50)值分别为 1.31 和 7.09 μg/mL。这些成分尚未在 L. fischeri 中报道过。