Department of Biomedical Engineering, University of California at Irvine, Irvine, CA 92697, USA.
J Colloid Interface Sci. 2010 Apr 15;344(2):521-7. doi: 10.1016/j.jcis.2010.01.042. Epub 2010 Jan 18.
Polymer-lipid microbubbles (PLBs) are generated by microfluidic flow-focusing devices to form a new class of long-lasting hybrid particles. The specific PLB construct developed is an elastic gas-filled microsphere with a polydimethylsiloxane (PDMS) shell containing phospholipids conjugated to functionalized polyethyleneglycol (PEG). Digital "droplet-based" microfluidics technology enables control of particle composition, size, and polydispersity (sigma<10%). Use of PDMS as a shell component improves the functionality and stability (lifetime>6 months) of the hybrid particles due to the thermally maneuverable solidification process. With a gas core, they serve as a template material for creating three-dimensional porous structures and surfaces, requiring no cumbersome post-processing removal steps. By adding biotinylated PEG-lipid derivatives that offer targeting capabilities, we demonstrate the immobilization of fluorescent IgG antibodies on stationary PDMS-lipid microbubbles through biotin-avidin interactions and on-chip trapping for immunoassays. A PDMS-lipid composition offers several advantages such as biocompatibility and biodegradability for future in vivo use as porous engineered scaffolds, packing materials, or delivery (e.g. therapeutic) agents with cell targeting capability.
聚合物-脂质微泡（PLB）是通过微流控流聚焦装置产生的，形成了一类新的长寿命混合颗粒。所开发的特定 PLB 结构是一种弹性充气微球，具有包含与官能化聚乙二醇（PEG）缀合的磷脂的聚二甲基硅氧烷（PDMS）壳。数字“基于液滴”的微流控技术能够控制颗粒的组成、大小和多分散性（sigma<10%）。由于热可操纵的固化过程，将 PDMS 用作壳成分可提高混合颗粒的功能和稳定性（寿命>6 个月）。由于具有气核，它们可用作用于创建三维多孔结构和表面的模板材料，不需要繁琐的后处理去除步骤。通过添加具有靶向能力的生物素化 PEG-脂质衍生物，我们通过生物素-亲和素相互作用和芯片上捕获，展示了荧光 IgG 抗体在固定化 PDMS-脂质微泡上的固定化，用于免疫分析。PDMS-脂质组成具有生物相容性和可生物降解性等优点，可用于未来作为多孔工程支架、包装材料或具有细胞靶向能力的递送（例如治疗）剂的体内应用。
