Demir Ramazan
Department of Histology and Embryology, Faculty of Medicine, Akdeniz University, Antalya, Turkey.
Folia Histochem Cytobiol. 2009 Jan;47(3):435-45. doi: 10.2478/v10042-009-0100-5.
The aim of this study was to determine the expression of VEGF and its receptors in placentas from normal pregnancies between 22 days p.c. and 48 days p.c. of very early pregnancy. Placental tissues carried out from 19 pregnant women were examined. Immunohistochemical technique, electron microscopy were employed to evaluate the factors expression. In the new developing mesenchymal villi and immature intermediate villi VEGF and its receptors VEGFR-1 and VEGFR-2 immunoreactivity was detected in all the placental components, while in the stem villi and in the chorionic plate with large vessels only in some components. In the mesenchymal villi and immature intermediate villi VEGFR-1 and -2, and angiopoietin receptors Tie-1 and -2 immunoreactivity was dominantly observed in the heamangiogenic cells and cells cords, whereas the matured villi showed immunoreactivity only in other components. The ultrastructural findings were higher in respect to the all of the early pregnancy days. The placental samples from all of pregnancies, showed the VEGF and its receptors in optimal expression levels, whereas the angiopoietin receptors Tie-1 and -2 showed a higher expression levels in respect to other study factors. The receptors protein levels increased from the early days to the advanced days of gestation, but this alteration was not significant. The intensity of the immunolabeling for these proteins were not significant compared to to each other of gestatin days were examined. These findings demonstrated that a dysregulation of the placental expression of the VEGF and its receptors related to the different degrees of the gestational periods. Probably, this event may be related to complete vasculugenesis and angiogenesis in placental villi.
本研究的目的是确定在妊娠第22天至第48天的极早期正常妊娠胎盘组织中血管内皮生长因子（VEGF）及其受体的表达情况。对19名孕妇的胎盘组织进行了检查。采用免疫组织化学技术和电子显微镜评估相关因子的表达。在新发育的间充质绒毛和未成熟的中间绒毛中，VEGF及其受体VEGFR-1和VEGFR-2的免疫反应性在所有胎盘成分中均有检测到，而在干绒毛和有大血管的绒毛膜板中，仅在部分成分中检测到。在间充质绒毛和未成熟的中间绒毛中，VEGFR-1和-2以及血管生成素受体Tie-1和-2的免疫反应性主要在血管生成细胞和细胞索中观察到，而成熟绒毛仅在其他成分中显示免疫反应性。超微结构发现在整个妊娠早期均较高。所有妊娠的胎盘样本中，VEGF及其受体均呈最佳表达水平，而血管生成素受体Tie-1和-2相对于其他研究因子显示出较高的表达水平。受体蛋白水平从妊娠早期到晚期有所增加，但这种变化不显著。在检查的不同妊娠天数之间，这些蛋白的免疫标记强度相互之间无显著差异。这些发现表明，VEGF及其受体在胎盘表达的失调与妊娠期的不同阶段有关。这一事件可能与胎盘绒毛中完全的血管发生和血管生成有关。
