基质辅助激光解吸电离飞行时间质谱法直接从阳性血培养颗粒中进行细菌鉴定。
Matrix-assisted laser desorption ionization-time of flight mass spectrometry for direct bacterial identification from positive blood culture pellets.
机构信息
Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland.
出版信息
J Clin Microbiol. 2010 Apr;48(4):1481-3. doi: 10.1128/JCM.01780-09. Epub 2010 Feb 17.
Abstract
An ammonium chloride erythrocyte-lysing procedure was used to prepare a bacterial pellet from positive blood cultures for direct matrix-assisted laser desorption-ionization time of flight (MALDI-TOF) mass spectrometry analysis. Identification was obtained for 78.7% of the pellets tested. Moreover, 99% of the MALDI-TOF identifications were congruent at the species level when considering valid scores. This fast and accurate method is promising.
摘要
采用氯化铵红细胞裂解程序从阳性血培养物中制备细菌沉淀，用于直接基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱分析。测试的沉淀中有 78.7% 得到了鉴定。此外，考虑到有效分数，当在种水平上考虑时，99% 的 MALDI-TOF 鉴定结果是一致的。这种快速准确的方法很有前途。
相似文献
1
Matrix-assisted laser desorption ionization-time of flight mass spectrometry for direct bacterial identification from positive blood culture pellets.基质辅助激光解吸电离飞行时间质谱法直接从阳性血培养颗粒中进行细菌鉴定。
J Clin Microbiol. 2010 Apr;48(4):1481-3. doi: 10.1128/JCM.01780-09. Epub 2010 Feb 17.
2
Microorganisms direct identification from blood culture by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.基质辅助激光解吸电离飞行时间质谱法直接从血培养物中鉴定微生物。
Clin Microbiol Infect. 2011 Apr;17(4):546-51. doi: 10.1111/j.1469-0691.2010.03257.x.
3
Use of MALDI-TOF MS technique for rapid identification of bacteria from positive blood cultures.使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）技术从阳性血培养物中快速鉴定细菌。
Indian J Med Microbiol. 2014 Oct-Dec;32(4):419-22. doi: 10.4103/0255-0857.142261.
4
Rapid identification of bacteria in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry.基质辅助激光解吸电离飞行时间质谱法快速鉴定阳性血培养肉汤中的细菌。
J Clin Microbiol. 2010 Feb;48(2):444-7. doi: 10.1128/JCM.01541-09. Epub 2009 Dec 2.
5
Direct identification of bacteria from positive BacT/ALERT blood culture bottles using matrix-assisted laser desorption ionization-time-of-flight mass spectrometry.使用基质辅助激光解吸电离飞行时间质谱法直接从阳性BacT/ALERT血培养瓶中鉴定细菌。
Diagn Microbiol Infect Dis. 2014 Nov;80(3):193-6. doi: 10.1016/j.diagmicrobio.2014.07.008. Epub 2014 Jul 31.
6
Rapid Identification of Bacteria Directly from Positive Blood Cultures by Use of a Serum Separator Tube, Smudge Plate Preparation, and Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry.通过使用血清分离管、涂抹板制备和基质辅助激光解吸电离飞行时间质谱直接从阳性血培养物中快速鉴定细菌
J Clin Microbiol. 2015 Oct;53(10):3349-52. doi: 10.1128/JCM.01493-15. Epub 2015 Jul 22.
7
Direct bacterial identification in positive blood cultures by use of two commercial matrix-assisted laser desorption ionization-time of flight mass spectrometry systems.使用两种商业化基质辅助激光解吸电离飞行时间质谱系统直接对阳性血培养物进行细菌鉴定。
J Clin Microbiol. 2013 Jun;51(6):1733-9. doi: 10.1128/JCM.03259-12. Epub 2013 Mar 20.
8
The effect of the blood culture bottle type in the rate of direct identification from positive cultures by matrix-assisted laser desorption/ionisation time-of-flight (MALDI-TOF) mass spectrometry.血培养瓶类型对通过基质辅助激光解吸/电离飞行时间（MALDI-TOF）质谱从阳性培养物中进行直接鉴定的速率的影响。
J Infect. 2011 Mar;62(3):251-3. doi: 10.1016/j.jinf.2010.12.008. Epub 2010 Dec 25.
9
Comparison of three preparatory methods for detection of bacteremia by MALDI-TOF mass spectrometry.比较三种 MALDI-TOF 质谱法检测菌血症的准备方法。
Diagn Microbiol Infect Dis. 2012 May;73(1):21-6. doi: 10.1016/j.diagmicrobio.2012.01.010.
10
Comparison of an in-house method and the commercial Sepsityper™ kit for bacterial identification directly from positive blood culture broths by matrix-assisted laser desorption-ionisation time-of-flight mass spectrometry.采用基质辅助激光解吸电离飞行时间质谱法直接从阳性血培养肉汤中进行细菌鉴定的内部方法与商业 Sepsityper™试剂盒的比较。
Eur J Clin Microbiol Infect Dis. 2012 Sep;31(9):2269-81. doi: 10.1007/s10096-012-1566-1. Epub 2012 Feb 25.
引用本文的文献
1
Direct Identification of Yeasts from Blood Cultures and Body Fluids Using MALDI-TOF MS with Concurrent Antifungal Susceptibility Testing.使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）直接从血培养物和体液中鉴定酵母菌并同时进行抗真菌药敏试验。
Mycopathologia. 2025 Aug 25;190(5):78. doi: 10.1007/s11046-025-00987-x.
2
Clinical evaluation of an in-house Xpert Lysate-based Method combined with MALDI-TOF MS for the rapid identification of positive blood cultures.基于内部Xpert裂解物法结合基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）对阳性血培养物进行快速鉴定的临床评估
BMC Microbiol. 2025 May 15;25(1):293. doi: 10.1186/s12866-025-03996-4.
3
Impact of rapid identification by MALDI-TOF MS from positive blood cultures in Enterococcus spp. bloodstream infections.基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）对阳性血培养物中肠球菌属血流感染进行快速鉴定的影响
Eur J Clin Microbiol Infect Dis. 2025 May;44(5):1185-1196. doi: 10.1007/s10096-025-05084-x. Epub 2025 Mar 8.
4
The impact of optimizing microbial diagnosis processes on clinical and healthcare economic outcomes in hospitalized patients with bloodstream infections.优化微生物诊断流程对住院血流感染患者的临床和医疗经济结局的影响。
Eur J Clin Microbiol Infect Dis. 2024 Nov;43(11):2147-2157. doi: 10.1007/s10096-024-04928-2. Epub 2024 Sep 6.
5
Rapid Direct Identification of Microbial Pathogens and Antimicrobial Resistance Genes in Positive Blood Cultures Using a Fully Automated Multiplex PCR Assay.采用全自动多重 PCR 检测法快速直接鉴定血培养中的微生物病原体和抗菌药物耐药基因。
J Korean Med Sci. 2024 May 6;39(17):e157. doi: 10.3346/jkms.2024.39.e157.
6
A Current Diagnostic and Therapeutic Challenge: Tinea Capitis.当前的诊断和治疗挑战：头癣
J Clin Med. 2024 Jan 10;13(2):376. doi: 10.3390/jcm13020376.
7
An improved protocol for bacteria identification by MALDI-TOF MS directly from positive blood cultures.一种改良的 MALDI-TOF MS 直接从阳性血培养物中进行细菌鉴定的方案。
Eur J Clin Microbiol Infect Dis. 2024 Mar;43(3):605-610. doi: 10.1007/s10096-023-04725-3. Epub 2023 Dec 19.
8
Evaluation of a sterile, filter-based, in-house method for rapid direct bacterial identification and antimicrobial susceptibility testing using positive blood culture.使用阳性血培养评估一种无菌、基于滤器的内部方法，用于快速直接细菌鉴定和抗菌药物敏感性测试。
Eur J Clin Microbiol Infect Dis. 2023 Jun;42(6):691-700. doi: 10.1007/s10096-023-04592-y. Epub 2023 Apr 3.
9
The Role of Coagulase-Negative Staphylococci Biofilms on Late-Onset Sepsis: Current Challenges and Emerging Diagnostics and Therapies.凝固酶阴性葡萄球菌生物膜在迟发性脓毒症中的作用：当前挑战及新兴诊断与治疗方法
Antibiotics (Basel). 2023 Mar 10;12(3):554. doi: 10.3390/antibiotics12030554.
10
Comparison of Accelerate PhenoTest BC Kit and MALDI-TOF MS/VITEK 2 System for the rapid identification and antimicrobial susceptibility testing of gram-negative bacilli causing bloodstream infections.比较Accelerate PhenoTest BC试剂盒与基质辅助激光解吸电离飞行时间质谱/MicroScan WalkAway微生物鉴定药敏分析系统用于快速鉴定和检测引起血流感染的革兰氏阴性杆菌的抗菌药敏性
J Assoc Med Microbiol Infect Dis Can. 2020 Oct 11;5(3):145-157. doi: 10.3138/jammi-2020-0004. eCollection 2020 Oct.
本文引用的文献
1
Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry.细菌学领域正在进行的变革：通过基质辅助激光解吸电离飞行时间质谱法对细菌进行常规鉴定。
Clin Infect Dis. 2009 Aug 15;49(4):543-51. doi: 10.1086/600885.
2
Evaluation of matrix-assisted laser desorption ionization-time-of-flight mass spectrometry in comparison to 16S rRNA gene sequencing for species identification of nonfermenting bacteria.基质辅助激光解吸电离飞行时间质谱与16S rRNA基因测序用于非发酵菌菌种鉴定的比较评估
J Clin Microbiol. 2008 Jun;46(6):1946-54. doi: 10.1128/JCM.00157-08. Epub 2008 Apr 9.
3
Highly efficient classification and identification of human pathogenic bacteria by MALDI-TOF MS.通过基质辅助激光解吸电离飞行时间质谱对人类致病细菌进行高效分类和鉴定
Mol Cell Proteomics. 2008 Feb;7(2):448-56. doi: 10.1074/mcp.M700339-MCP200. Epub 2007 Nov 27.
4
Rapid identification of viridans streptococci by mass spectrometric discrimination.通过质谱鉴别快速鉴定草绿色链球菌
J Clin Microbiol. 2007 Aug;45(8):2392-7. doi: 10.1128/JCM.00556-07. Epub 2007 Jun 6.
5
Detection and treatment of bloodstream infection: laboratory reporting and antimicrobial management.血流感染的检测与治疗：实验室报告与抗菌药物管理
J Clin Microbiol. 2003 Jan;41(1):495-7. doi: 10.1128/JCM.41.1.495-497.2003.
6
Sample preparation of Gram-positive bacteria for identification by matrix assisted laser desorption/ionization time-of-flight.用于通过基质辅助激光解吸/电离飞行时间进行鉴定的革兰氏阳性菌样本制备
J Microbiol Methods. 2002 Feb;48(2-3):107-15. doi: 10.1016/s0167-7012(01)00315-3.
7
Phyloproteomics: species identification of Enterobacteriaceae using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.系统发育蛋白质组学：使用基质辅助激光解吸/电离飞行时间质谱法对肠杆菌科进行物种鉴定。
J Mol Microbiol Biotechnol. 2001 Jan;3(1):103-12.
Zotero 插件