传统表型鉴定方法与部分 5' 16S rRNA 基因测序在非发酵革兰阴性杆菌种水平鉴定中的比较。
Comparison of traditional phenotypic identification methods with partial 5' 16S rRNA gene sequencing for species-level identification of nonfermenting Gram-negative bacilli.
机构信息
ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah 84108, USA.
出版信息
J Clin Microbiol. 2010 Apr;48(4):1442-4. doi: 10.1128/JCM.00169-10. Epub 2010 Feb 17.
Abstract
Correct identification of nonfermenting Gram-negative bacilli (NFB) is crucial for patient management. We compared phenotypic identifications of 96 clinical NFB isolates with identifications obtained by 5' 16S rRNA gene sequencing. Sequencing identified 88 isolates (91.7%) with >99% similarity to a sequence from the assigned species; 61.5% of sequencing results were concordant with phenotypic results, indicating the usability of sequencing to identify NFB.
摘要
正确鉴定非发酵革兰氏阴性杆菌（NFB）对于患者管理至关重要。我们比较了 96 株临床 NFB 分离株的表型鉴定与 5' 16S rRNA 基因测序鉴定的结果。测序鉴定出 88 株（91.7%）与指定种属的序列具有>99%的相似性；测序结果与表型结果的符合率为 61.5%，表明测序可用于鉴定 NFB。
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