Department of Molecular Genetics, The Forsyth Institute, 140 The Fenway, Boston, MA 02115, USA.
J Dent Res. 2010 Apr;89(4):378-83. doi: 10.1177/0022034509360010. Epub 2010 Feb 17.
Dental caries in pre-school children has significant public health and health disparity implications. To determine microbial risk markers for this infection, this study aimed to compare the microbiota of children with early childhood caries with that of caries-free children. Plaque samples from incisors, molars, and the tongue from 195 children attending pediatricians' offices were assayed by 74 DNA probes and by PCR to Streptococcus mutans. Caries-associated factors included visible plaque, child age, race, and snacking habits. Species were detected more frequently from tooth than tongue samples. Lactobacillus gasseri (p < 0.01), Lactobacillus fermentum, Lactobacillus vaginalis, and S. mutans with Streptococcus sobrinus (all p < 0.05) were positively associated with caries. By multifactorial analysis, the probiotic Lactobacillus acidophilus was negatively associated with caries. Prevotella nigrescens was the only species (p < 0.05) significantly associated with caries by the 'false discovery' rate. Analysis of the data suggests that selected Lactobacillus species, in addition to mutans streptococci, are risk markers for early childhood caries.
学龄前儿童的龋齿问题具有重要的公共卫生和健康差异意义。为了确定这种感染的微生物风险标志物，本研究旨在比较患有幼儿龋齿和无龋齿儿童的口腔微生物群。通过 74 个 DNA 探针和聚合酶链反应（PCR），对 195 名在儿科医生办公室就诊的儿童的切牙、磨牙和舌头上的菌斑样本进行了检测。龋齿相关因素包括可见菌斑、儿童年龄、种族和零食习惯。与舌样本相比，从牙齿样本中检测到的物种更频繁。与龋齿呈正相关的有乳杆菌属（p<0.01）、发酵乳杆菌、阴道乳杆菌和变异链球菌与远缘链球菌（均 p<0.05）。通过多因素分析，益生菌嗜酸乳杆菌与龋齿呈负相关。普雷沃氏菌属（p<0.05）是唯一通过“假发现率”与龋齿显著相关的物种。数据分析表明，除变形链球菌外，某些乳杆菌属物种也是幼儿龋齿的风险标志物。
