Sánchez Manuel, Colom Francisca
Grupo BSM (Biotecnología, Salud y Medio Ambiente), Departamento de Producción Vegetal y Microbiología, Universidad Miguel Hernández, Alicante, Spain.
Rev Iberoam Micol. 2010 Mar 31;27(1):10-3. doi: 10.1016/j.riam.2009.11.004. Epub 2010 Feb 18.
Extracellular DNase activity was studied in 73 strains of Cryptococcus neoformans and 12 strains of Cryptococcus gattii. DNase activity was measured by DNase agar clearance with and without Methyl Green. All strains tested showed extracellular DNase activity and no significant difference was found betweenC. neoformans and C. gattii strains. DNase production was higher in strains from clinical origin (average radius of 6.2 mm) than among environmental strains (average radius of 2.9 mm). The extracellular enzyme may be detected by DNA substrate PAGE assays and its molecular weight was estimated at 31 kD. These results suggest that extracellular DNase could be considered as a virulence factor involved in C. neoformans-C. gattii species complex pathogenicity.
对73株新型隐球菌和12株格特隐球菌的细胞外DNA酶活性进行了研究。通过在有和没有甲基绿的情况下用DNA酶琼脂清除法来测量DNA酶活性。所有测试菌株均显示出细胞外DNA酶活性，新型隐球菌和格特隐球菌菌株之间未发现显著差异。临床来源菌株的DNA酶产量（平均半径为6.2毫米）高于环境菌株（平均半径为2.9毫米）。细胞外酶可通过DNA底物PAGE分析检测到，其分子量估计为31 kD。这些结果表明，细胞外DNA酶可被视为参与新型隐球菌-格特隐球菌复合种致病性的一种毒力因子。
