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慢性感染细胞系中未整合的1型人类免疫缺陷病毒DNA与表面CD4表达无关。

Unintegrated human immunodeficiency virus type 1 DNA in chronically infected cell lines is not correlated with surface CD4 expression.

作者信息

Besansky N J, Butera S T, Sinha S, Folks T M

机构信息

Retrovirus Diseases Branch, Centers for Disease Control, Atlanta, Georgia 30333.

出版信息

J Virol. 1991 May;65(5):2695-8. doi: 10.1128/JVI.65.5.2695-2698.1991.

DOI:10.1128/JVI.65.5.2695-2698.1991
PMID:2016776
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC240630/
Abstract

Using a polymerase chain reaction-based assay on total cell lysates, we have detected unintegrated human immunodeficiency virus type 1 (HIV-1) DNA in chronically infected T-lymphocytic (ACH-2, J1) and promyelocytic (OM-10.1) cell lines. Treatment with 3'-azido-3'-deoxythymidine (AZT) or soluble CD4 inhibited accumulation of unintegrated viral DNA about 10-fold within 72 h; removal of AZT permitted recovery to pretreatment levels within 72 h. Our results indicate that unintegrated HIV-1 DNA is unstable in these cell lines and originates from a continuous process of reinfection. OM-10.1 cells had relatively high levels of surface CD4 by flow cytometry and high levels of unintegrated viral DNA by polymerase chain reaction. ACH-2 cells had very low levels of both surface CD4 and unintegrated viral DNA. However, J1 cells, with surface CD4 below the level of detection of flow cytometry had a high level of unintegrated viral DNA similar to that of OM-10.1 cells. This implies that the number of CD4 receptors is not rate limiting for reinfection.

摘要

通过对全细胞裂解物进行基于聚合酶链反应的检测，我们在慢性感染的T淋巴细胞系（ACH-2、J1）和早幼粒细胞系（OM-10.1）中检测到了未整合的1型人类免疫缺陷病毒（HIV-1）DNA。用3'-叠氮-3'-脱氧胸苷（AZT）或可溶性CD4处理可在72小时内将未整合病毒DNA的积累抑制约10倍；去除AZT可使病毒DNA在72小时内恢复到预处理水平。我们的结果表明，未整合的HIV-1 DNA在这些细胞系中不稳定，且源自持续的再感染过程。通过流式细胞术检测，OM-10.1细胞表面CD4水平相对较高，通过聚合酶链反应检测其未整合病毒DNA水平也较高。ACH-2细胞表面CD4和未整合病毒DNA水平都非常低。然而，J1细胞表面CD4低于流式细胞术的检测水平，但其未整合病毒DNA水平却与OM-10.1细胞相似，很高。这意味着CD4受体的数量不是再感染的限速因素。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c55b/240630/ee2eed39416b/jvirol00048-0554-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c55b/240630/1a3b8580c9b5/jvirol00048-0554-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c55b/240630/ee2eed39416b/jvirol00048-0554-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c55b/240630/1a3b8580c9b5/jvirol00048-0554-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c55b/240630/ee2eed39416b/jvirol00048-0554-b.jpg

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本文引用的文献

1
Correlation between cell killing and massive second-round superinfection by members of some subgroups of avian leukosis virus.禽白血病病毒某些亚群成员的细胞杀伤与大规模第二轮超级感染之间的相关性。
J Virol. 1980 Jan;33(1):494-506. doi: 10.1128/JVI.33.1.494-506.1980.
2
Structure of the human immune interferon gene.人类免疫干扰素基因的结构。
Nature. 1982 Aug 26;298(5877):859-63. doi: 10.1038/298859a0.
3
The CD4 (T4) antigen is an essential component of the receptor for the AIDS retrovirus.CD4（T4）抗原是艾滋病逆转录病毒受体的重要组成部分。
潜伏感染细胞系中的HIV整合位点：持续复制的证据。
Retrovirology. 2017 Jan 13;14(1):2. doi: 10.1186/s12977-016-0325-2.
4
Adenosine deaminase acting on RNA-1 (ADAR1) inhibits HIV-1 replication in human alveolar macrophages.作用于RNA-1的腺苷脱氨酶（ADAR1）抑制HIV-1在人肺泡巨噬细胞中的复制。
PLoS One. 2014 Oct 1;9(10):e108476. doi: 10.1371/journal.pone.0108476. eCollection 2014.
5
Viral complementation allows HIV-1 replication without integration.病毒互补作用可使HIV-1在不整合的情况下进行复制。
Retrovirology. 2008 Jul 9;5:60. doi: 10.1186/1742-4690-5-60.
6
Nonintegrated circular forms of the HIV-1 provirus DNA in experimental HIV infection.实验性HIV感染中HIV-1前病毒DNA的非整合环状形式
Dokl Biochem Biophys. 2001 Mar-Apr;377:116-8. doi: 10.1023/a:1011596110694.
7
High susceptibility of U937-derived subclones to human immunodeficiency virus type 1 infection correlates with accumulation of unintegrated circular viral DNA.U937衍生亚克隆对1型人类免疫缺陷病毒感染的高易感性与未整合环状病毒DNA的积累相关。
Virus Genes. 1996;12(2):117-29. doi: 10.1007/BF00572950.
8
Pathogenesis of human immunodeficiency virus infection.人类免疫缺陷病毒感染的发病机制
Microbiol Rev. 1993 Mar;57(1):183-289. doi: 10.1128/mr.57.1.183-289.1993.
9
Infection of macrophages with lymphotropic human immunodeficiency virus type 1 can be arrested after viral DNA synthesis.用亲淋巴细胞性1型人类免疫缺陷病毒感染巨噬细胞后，病毒DNA合成后感染可被阻止。
J Virol. 1993 Nov;67(11):6893-6. doi: 10.1128/JVI.67.11.6893-6896.1993.
10
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Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6405-9. doi: 10.1073/pnas.90.14.6405.
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4
Downregulation of cell surface molecules during noncytopathic infection of T cells with human immunodeficiency virus.人免疫缺陷病毒对T细胞进行非细胞病变感染期间细胞表面分子的下调
J Virol. 1987 Dec;61(12):3741-8. doi: 10.1128/JVI.61.12.3741-3748.1987.
5
DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells.用于直接检测外周血单个核细胞DNA中HIV-1的DNA扩增。
Science. 1988 Jan 15;239(4837):295-7. doi: 10.1126/science.3336784.
6
The human immunodeficiency virus: infectivity and mechanisms of pathogenesis.人类免疫缺陷病毒：传染性与发病机制
Science. 1988 Feb 5;239(4840):617-22. doi: 10.1126/science.3277274.
7
Alterations in T4 (CD4) protein and mRNA synthesis in cells infected with HIV.感染HIV的细胞中T4（CD4）蛋白及mRNA合成的改变。
Science. 1986 Nov 28;234(4780):1123-7. doi: 10.1126/science.3095925.
8
Biological and biochemical characterization of a cloned Leu-3- cell surviving infection with the acquired immune deficiency syndrome retrovirus.一株感染获得性免疫缺陷综合征逆转录病毒后存活的克隆化Leu-3-细胞的生物学及生化特性
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9
Disease-specific and tissue-specific production of unintegrated feline leukaemia virus variant DNA in feline AIDS.猫艾滋病中未整合的猫白血病病毒变异体DNA的疾病特异性和组织特异性产生
Nature. 1986;319(6051):333-6. doi: 10.1038/319333a0.
10
Characterization of a continuous T-cell line susceptible to the cytopathic effects of the acquired immunodeficiency syndrome (AIDS)-associated retrovirus.一种对获得性免疫缺陷综合征（AIDS）相关逆转录病毒的细胞病变效应敏感的连续T细胞系的特性分析。
Proc Natl Acad Sci U S A. 1985 Jul;82(13):4539-43. doi: 10.1073/pnas.82.13.4539.