Zoology Department, University School of Sciences, Gujarat University, Navrangpura, Ahmedabad, India.
Food Chem Toxicol. 2010 May;48(5):1234-8. doi: 10.1016/j.fct.2010.02.015. Epub 2010 Feb 17.
The present study was aimed to evaluate curcumin as a potential natural antioxidant to mitigate the genotoxic effects of arsenic (As) and fluoride (F) in human peripheral blood lymphocytes. The study was divided into nine groups consisting of negative control, positive control treated with ethyl methane sulphonate (EMS; 1.93 mM) and curcumin control with only curcumin (1.7 microM) in blood culture. As (1.4 microM) and F (34 microM) were added alone as well as in combination, to the cultures, with and without curcumin. Cultures were analysed for chromosomal aberrations (both structural and numerical) and primary DNA damage via comet assay as the genotoxic parameters after an exposure duration of 24h. Results revealed that curcumin efficiently ameliorates the toxic effect of As and F by reducing the frequency of structural aberrations (>60%), hypoploidy (>50%) and primary DNA damage. In conclusion, curcumin mitigates the genotoxic effects of the two well known water contaminants (As and F) effectively and efficiently at the given concentration in vitro.
本研究旨在评估姜黄素作为一种潜在的天然抗氧化剂，以减轻砷（As）和氟化物（F）对人外周血淋巴细胞的遗传毒性作用。该研究分为九组，包括阴性对照组、用乙基亚磺酸钠（EMS；1.93mM）处理的阳性对照组和仅用姜黄素（1.7μM）处理的姜黄素对照组。As（1.4μM）和 F（34μM）单独添加以及组合添加到培养物中，同时添加和不添加姜黄素。在 24 小时暴露后，通过染色体畸变（结构和数量）和彗星试验分析作为遗传毒性参数来分析培养物中的原发性 DNA 损伤。结果表明，姜黄素通过降低结构畸变（>60%）、亚二倍体（>50%）和原发性 DNA 损伤的频率，有效地减轻了 As 和 F 的毒性作用。总之，姜黄素在体外以给定浓度有效地减轻了两种已知的水污染物（As 和 F）的遗传毒性作用。
