Department of Dental Sciences, University of Bologna, Via San Vitale 59, 40139 Bologna, Italy.
Biomaterials. 2010 May;31(13):3527-35. doi: 10.1016/j.biomaterials.2010.01.066. Epub 2010 Feb 18.
Previous in vivo studies have shown a limited potential for vertical bone regeneration using osteoconductive scaffolds alone. In the present study, we investigated whether the association of adipose-derived adult stem cells (ASCs) with anorganic bovine bone (ABB) scaffold improved bone formation and implant osseointegration in a vertical guided bone regeneration model. Two pre-formed titanium domes were placed on the calvaria of 12 rabbits. Four treatment modalities were evenly distributed among the 24 domes: ABB alone, and ABB containing 3 x 10(5), 3 x 10(6), or 3 x 10(7) cells/graft. After 1 month, the domes were removed and one titanium implant was placed into each augmented site. One month after the second operation, the animals were killed and biopsy specimens were examined by histomorphometric and micro-CT analyses. Results indicated that at all concentrations, the ASC-loaded groups showed significantly more new bone formation and higher mean values of bone-implant contact and bone density inside threads than the ABB group. Furthermore, ASCs demonstrated a dose-response relationship, with the highest dose chosen inducing more robust bone regeneration. This study suggests that the delivery of ASCs on ABB might effectively increase vertical bone regeneration and implant osseointegration, versus ABB alone.
先前的体内研究表明，单独使用骨诱导支架的垂直骨再生潜力有限。在本研究中，我们研究了脂肪源性成体干细胞（ASCs）与无机组分牛骨（ABB）支架联合应用是否能改善垂直引导骨再生模型中的骨形成和种植体骨整合。将两个预制钛穹顶放置在 12 只兔子的颅骨上。24 个穹顶均匀分布 4 种治疗方式：单独使用 ABB，以及每克移植物中分别含有 3 x 10(5)、3 x 10(6)或 3 x 10(7)个细胞的 ABB。1 个月后，取出穹顶，并将一个钛植入物放入每个增强部位。第二次手术后 1 个月，处死动物，通过组织形态计量学和 micro-CT 分析检查活检标本。结果表明，在所有浓度下，负载 ASC 的组显示出明显更多的新骨形成，以及更高的种植体-骨接触和螺纹内骨密度的平均值，高于 ABB 组。此外，ASCs 表现出剂量反应关系，所选的最高剂量诱导出更强大的骨再生。这项研究表明，与单独使用 ABB 相比，将 ASC 递送至 ABB 可能有效增加垂直骨再生和种植体骨整合。
