Department of Meteorology, Stockholm University, Svante Arrhenius vag 12, SE-10691 Stockholm, Sweden.
Anal Chim Acta. 2010 Mar 10;662(2):193-9. doi: 10.1016/j.aca.2010.01.008. Epub 2010 Jan 13.
A method was established using capillary electrophoresis with indirect UV detection for analysis of monosaccharides liberated from exopolysaccharides by acidic hydrolysis. Tangential flow filtration was used to isolate high molecular weight polysaccharides from seawater. The capillary electrophoresis method included the use of a background electrolyte consisting of 2,6-dimethoxyphenol and cetyltrimethylammonium bromide. Several neutral sugars commonly existing in marine polysaccharides were separated under optimized conditions. The relative standard deviations were between 1.3% and 2.3% for relative migration time and 1.3-2.5% for peak height. Detection limits (at S/N 3) were in the range of 27.2-47.8 microM. The proposed approach was applied to the analysis of hydrolyzed colloidal polysaccharides in seawater collected from the Baltic Sea. Nanomolar levels of liberated monosaccharides in seawater samples can be detected by preconcentration up to 30,000 times.
建立了一种采用毛细管电泳间接紫外检测法分析酸性水解后从胞外多糖中释放出的单糖的方法。切向流过滤用于从海水中分离高分子量多糖。毛细管电泳法使用由 2,6-二甲氧基苯酚和十六烷基三甲基溴化铵组成的背景电解质。在优化条件下，分离出了海洋多糖中常见的几种中性糖。相对迁移时间的相对标准偏差为 1.3%至 2.3%，峰高的相对标准偏差为 1.3-2.5%。检测限（S/N 为 3）在 27.2-47.8μM 范围内。该方法应用于分析波罗的海采集的海水中水解胶体多糖。通过预浓缩可将海水中释放的单糖的检出限提高到纳摩尔水平，浓缩倍数高达 30000 倍。
