Cancer Biology Proteomics Group, Postgraduate Medical Institute of the University of Hull, Hull, UK.
Cancer Lett. 2010 Aug 1;294(1):13-24. doi: 10.1016/j.canlet.2010.01.036. Epub 2010 Feb 21.
This review describes and discusses the advantages and limitations of proteomic approaches in the identification of biomarkers associated with chemotherapy resistance. Both gel-based (two-dimensional polyacrylamide gel electrophoresis) and gel-free (shotgun and quantitative) mass spectrometry approaches are discussed. Non-mass spectrometry approaches including antibody microarray platforms are described as complementary proteomic strategies. Methods for technical confirmation and clinical validation of putative biomarkers are presented. Use of this proteomic toolbox in the quest for biomarkers of chemotherapy resistance in breast cancer is reviewed. Technical aspects of sample selection, acquisition, storage and analysis are discussed and putative biomarkers identified through proteomic approaches are presented.
本文综述了蛋白质组学方法在鉴定与化疗耐药相关的生物标志物方面的优势和局限性。讨论了基于凝胶（二维聚丙烯酰胺凝胶电泳）和无凝胶（鸟枪法和定量）质谱法的方法。还描述了包括抗体微阵列平台在内的非质谱法方法，将其作为互补的蛋白质组学策略。介绍了用于技术确认和临床验证候选生物标志物的方法。综述了在乳腺癌中寻找化疗耐药生物标志物的过程中使用这种蛋白质组学工具的情况。讨论了样品选择、采集、储存和分析的技术方面，并提出了通过蛋白质组学方法鉴定的候选生物标志物。
