Issa Yasmine Amr, Ooda Said Ahmed, Salem Aziza Ibrahim, Idris Sahar Nasr, Elderbawy Mona Mohammed, Tolba Mona Mohamed
Department of Medical Biochemistry, Faculty of Medicine, University of Alexandria, Egypt.
College of Medicine, Arab Academy of Science Technology and Maritime Transport, Egypt.
Trop Parasitol. 2023 Jan-Jun;13(1):46-53. doi: 10.4103/tp.tp_28_22. Epub 2023 May 19.
sp. is the most common parasitic infestation in humans. However, its pathogenicity remains controversial. Our aim was to study the prevalence of sp. parasite subtypes in patients with gastrointestinal manifestations referred for colonoscopy and assess possible correlation with clinical, colonoscopic, and histopathological findings.
One hundred patients with gastrointestinal manifestations referred for colonoscopy were enrolled. Stool samples were collected and examined both microscopically and by real-time quantitative polymerase chain reaction (qPCR) for detection of sp. Subtyping was done for positive samples by qPCR and confirmed by sequencing.
qPCR sensitivity far exceeded microscopy in detection of sp. (58% vs. 31%, agreement 38.5%). The most commonly detected subtype was 3 (50%), followed by 2 (32.8%) and 4 (13.8%). Abdominal pain was the most common clinical symptom; inflammation and colitis were the most common abnormal colonoscopic and histopathological findings. The most frequent subtype encountered in those findings was Subtype 3.
This study confirmed the importance of using qPCR in diagnosis of sp. An association between abnormal clinical, colonoscopic, and histopathological findings on the one hand, and sp. infestation, especially Subtype 3, on the other hand, is also posed. This necessitates further studies to assess the mechanism of association with pathogenicity.
[寄生虫名称]是人类最常见的寄生虫感染。然而，其致病性仍存在争议。我们的目的是研究因结肠镜检查转诊的有胃肠道症状患者中[寄生虫名称]寄生虫亚型的流行情况，并评估其与临床、结肠镜检查及组织病理学结果之间可能存在的相关性。
纳入100例因结肠镜检查转诊的有胃肠道症状的患者。采集粪便样本，通过显微镜检查和实时定量聚合酶链反应（qPCR）检测[寄生虫名称]。对阳性样本进行qPCR亚型分析，并通过测序进行确认。
在检测[寄生虫名称]方面，qPCR的敏感性远远超过显微镜检查（58%对31%，一致性为38.5%）。最常检测到的亚型是3型（50%），其次是2型（32.8%）和4型（13.8%）。腹痛是最常见的临床症状；炎症和结肠炎是最常见的结肠镜检查及组织病理学异常结果。在这些结果中最常遇到的亚型是3型。
本研究证实了使用qPCR诊断[寄生虫名称]的重要性。一方面，临床、结肠镜检查及组织病理学异常结果与另一方面的[寄生虫名称]感染，尤其是3型感染之间也存在关联。这需要进一步研究以评估与致病性相关的机制。
