Department of Food Science, Cornell University, Ithaca, New York 14853, USA.
Foodborne Pathog Dis. 2010 Jun;7(6):707-17. doi: 10.1089/fpd.2009.0424.
Pulsed-field gel electrophoresis (PFGE) characterization of 335 temporally and spatially matched clinical, bovine, and human Salmonella enterica subsp. enterica isolates revealed 167 XbaI PFGE patterns. These isolates were previously classified into 51 serotypes and 73 sequence types, as determined by multilocus sequence typing. Discriminatory power of PFGE (Simpson's index, D = 0.991) was considerably higher than that of multilocus sequence typing (D = 0.920) or serotyping (D = 0.913). Although 128 PFGE types each only represented a single isolate, 8 PFGE types represented >4 isolates, including (i) three serotype Enteritidis and Heidelberg patterns that were only identified among human isolates, (ii) two PFGE patterns (each representing serotypes Bardo and Newport) that were significantly more common among bovine isolates as compared with human isolates; (iii) two PFGE types that each includes two serotypes (4,5,12:i:- and Typhimurium; Thompson and 1,7:-:1,5); and (iv) one PFGE type that includes eight Typhimurium isolates from humans and cattle. Characterization of isolates collected over multiple farm visits indicated that given specific PFGE types persisted over time on 11 farms. On an additional seven farms, isolates with a given sequence type represented multiple PFGE type, which typically only differed by <3 bands, suggesting PFGE type diversification during strain persistence. Sixteen PFGE types were isolated from 2 or more farms, including two widely distributed serotype Newport-associated PFGE types each found on 10 farms. In six instances two or three human isolates collected in the same county in the same or consecutive months represented the same subtypes, suggesting small human case clusters. PFGE-based characterization and surveillance of human and animal isolates can provide improved understanding of Salmonella diversity and epidemiology, including identification of possible host-associated and common, widely distributed PFGE types.
脉冲场凝胶电泳（PFGE）对 335 份时间和空间匹配的临床、牛和人源沙门氏菌肠亚种分离株的特征分析显示，有 167 种 XbaI PFGE 图谱。这些分离株先前通过多位点序列分型被分为 51 种血清型和 73 种序列型。PFGE 的分辨力（辛普森指数，D = 0.991）明显高于多位点序列分型（D = 0.920）或血清分型（D = 0.913）。尽管 128 种 PFGE 型各自仅代表单个分离株，但 8 种 PFGE 型代表了>4 个分离株，包括（i）仅在人源分离株中发现的 3 种肠炎血清型和 Heidelberg 型，（ii）两种 PFGE 型（分别代表 Bardo 和 Newport 血清型）在牛源分离株中比人源分离株更为常见；（iii）两种 PFGE 型各自包含两种血清型（4,5,12:i:-和 Typhimurium；Thompson 和 1,7:-:1,5）；以及（iv）一种 PFGE 型包含来自人源和牛源的 8 株 Typhimurium 分离株。对多次农场访问期间采集的分离株的特征分析表明，在 11 个农场中，特定的 PFGE 型在时间上持续存在。在另外 7 个农场中，具有特定序列型的分离株代表了多种 PFGE 型，这些 PFGE 型通常仅相差<3 条带，这表明在菌株持续存在期间，PFGE 型发生了多样化。16 种 PFGE 型从 2 个或更多农场分离，包括 2 种广泛分布的与血清型 Newport 相关的 PFGE 型，分别在 10 个农场中发现。在 6 个实例中，在同一县、同一或连续数月采集的 2 或 3 个人类分离株代表了相同的亚型，表明存在较小的人类病例群。基于 PFGE 的人类和动物分离株的特征分析和监测可以提供对沙门氏菌多样性和流行病学的更好理解，包括鉴定可能与宿主相关的和常见的、广泛分布的 PFGE 型。
