Govindarajan Raghavan, Rastogi Subha, Vijayakumar Madhavan, Shirwaikar Annie, Rawat Ajay Kumar Singh, Mehrotra Shanta, Pushpangadan Palpu
Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute, India.
Biol Pharm Bull. 2003 Oct;26(10):1424-7. doi: 10.1248/bpb.26.1424.
Desmodium gangeticum is herbal species which is widely used in the indigenous system of medicine and is reported to contain flavone and isoflavanoid glycosides. In view of its wide use and it's chemical composition, this study was aimed at examining the antioxidant activity of the extract of D. gangeticum. The extract was studied for diphenyl picryl hydrazyl (DPPH), nitric oxide, ferryl-bipyridyl and hypochlorous acid scavenging activity along with lipid peroxidation. Nitric oxide was generated using sodium nitroprusside and was studied using Griess reagent. In order to study the iron chelating capacity of the extract, the percentage ferryl-bipyridyl inhibition was studied. Hypochlorous acid scavenging activity was tested by measuring the inhibition of 5-thio-2-nitrobenzoic acid oxidation. The extract was also studied for lipid peroxidation assay by thiobarbituric acid-reactive substances (TBARS) method using rat brain homogenate. The results indicate that D. gangeticum extract has potent antioxidant activity.
阔叶山蚂蝗是一种草本植物，在传统医学体系中被广泛使用，据报道含有黄酮和异黄酮糖苷。鉴于其广泛应用及其化学成分，本研究旨在检测阔叶山蚂蝗提取物的抗氧化活性。对该提取物进行了二苯基苦味酰基自由基（DPPH）、一氧化氮、高铁-联吡啶和次氯酸清除活性以及脂质过氧化的研究。一氧化氮通过硝普钠产生，并使用格里斯试剂进行研究。为了研究提取物的铁螯合能力，研究了高铁-联吡啶抑制百分比。通过测量对5-硫代-2-硝基苯甲酸氧化的抑制来测试次氯酸清除活性。还使用大鼠脑匀浆通过硫代巴比妥酸反应性物质（TBARS）方法对该提取物进行脂质过氧化测定研究。结果表明，阔叶山蚂蝗提取物具有强大的抗氧化活性。
