George S, Chaturvedi P
Department of Biological Sciences, University of Botswana, Gaborone, Botswana.
Br J Biomed Sci. 2008;65(2):80-5. doi: 10.1080/09674845.2008.11732802.
Ethanol is the most frequently abused drug and causes a variety of pathological disturbances. It causes toxicity to tissues by generating free radicals during the course of its metabolism that can damage cellular structure and function, especially in hepatocytes. This study investigates the preventive and protective effects of Ocimum canum on alcohol-induced oxidative stress. Male Wistar rats were used in three separate experiments. First, two groups of six rats each (normal control and alcohol-treated) were used to establish hepatotoxicity. The alcohol-treated group showed a significant increase in TBARS and decreased activities of SOD, catalase, GSH, alpha-tocopherol and ascorbic acid. Second, the preventive effect of the O. canum extract was assessed. Four groups of rats (six in each group) were used and the experimental groups were treated with ethanol and graded doses of the extract for four weeks. Normal control and alcohol-treated groups were also assessed. Lipid peroxidation and antioxidant potential were quantified in plasma samples, which showed that the extract had a preventive effect. Third, the curative effect of the extract was assessed. The rats were divided into four groups comprising a normal control group on a normal diet and three other groups given alcohol for four weeks to establish alcohol toxicity. One of the alcohol groups was used as a control and the other two alcohol groups were given graded doses of the extract. After four weeks the rats were sacrificed in order to assess the lipid peroxidation and antioxidant potentials. The results indicated that the O. canum extract had hepatoprotective abilities against alcohol-induced oxidative stress.
乙醇是最常被滥用的药物，会引发多种病理紊乱。它在代谢过程中通过产生自由基对组织造成毒性，这些自由基会损害细胞结构和功能，尤其是在肝细胞中。本研究调查了毛罗勒对酒精诱导的氧化应激的预防和保护作用。雄性Wistar大鼠用于三个独立实验。首先，两组各六只大鼠（正常对照组和酒精处理组）用于建立肝毒性。酒精处理组的丙二醛显著增加，超氧化物歧化酶、过氧化氢酶、谷胱甘肽、α-生育酚和抗坏血酸的活性降低。其次，评估了毛罗勒提取物的预防作用。使用四组大鼠（每组六只），实验组用乙醇和不同剂量的提取物处理四周。同时也评估了正常对照组和酒精处理组。对血浆样本中的脂质过氧化和抗氧化潜力进行了量化，结果表明该提取物具有预防作用。第三，评估了提取物的治疗效果。大鼠被分为四组，一组为正常饮食的正常对照组，其他三组给予酒精四周以建立酒精毒性。其中一组酒精处理组作为对照组，另外两组酒精处理组给予不同剂量的提取物。四周后处死大鼠以评估脂质过氧化和抗氧化潜力。结果表明，毛罗勒提取物对酒精诱导的氧化应激具有肝脏保护能力。
