Centre de coopération Internationale en Recherche Agronomique pour le Développement (CIRAD), UMR 1098 Plant Development and Molecular Improvement, Avenue Agropolis, TA 03/96, 34398, Montpellier Cedex 5, France.
Theor Appl Genet. 2010 May;120(8):1673-87. doi: 10.1007/s00122-010-1284-y. Epub 2010 Feb 25.
A quantitative trait locus (QTL) analysis designed for a multi-parent population was carried out and tested in oil palm (Elaeis guineensis Jacq.), which is a diploid cross-fertilising perennial species. A new extension of the MCQTL package was especially designed for crosses between heterozygous parents. The algorithm, which is now available for any allogamous species, was used to perform and compare two types of QTL search for small size families, within-family analysis and across-family analysis, using data from a 2 x 2 complete factorial mating experiment involving four parents from three selected gene pools. A consensus genetic map of the factorial design was produced using 251 microsatellite loci, the locus of the Sh major gene controlling fruit shell presence, and an AFLP marker of that gene. A set of 76 QTLs involved in 24 quantitative phenotypic traits was identified. A comparison of the QTL detection results showed that the across-family analysis proved to be efficient due to the interconnected families, but the family size issue is just partially solved. The identification of QTL markers for small progeny numbers and for marker-assisted selection strategies is discussed.
进行了一项针对多亲本群体的数量性状基因座（QTL）分析，并在油棕（Elaeis guineensis Jacq.）中进行了测试，油棕是一种二倍体异交多年生物种。专门为杂合亲本之间的杂交设计了 MCQTL 包的新扩展。该算法现在可用于任何异花授粉物种，用于使用来自涉及三个选定基因库的四个亲本的 2 x 2 完全因子交配实验的数据，对小家族进行两种类型的 QTL 搜索，即家内分析和家间分析。使用 251 个微卫星标记、控制果实壳存在的 Sh 主要基因的位点和该基因的 AFLP 标记，生成了因子设计的共识遗传图谱。鉴定出了与 24 个数量表型性状相关的 76 个 QTL。对 QTL 检测结果的比较表明，由于家族之间的相互联系，家间分析被证明是有效的，但家族规模问题只是部分解决了。讨论了用于小后代数量的 QTL 标记和用于标记辅助选择策略的 QTL 标记的鉴定。
