Faculty of Agriculture in Osijek, Osijek, Croatia.
Mycopathologia. 2010 Jul;170(1):51-60. doi: 10.1007/s11046-010-9289-2. Epub 2010 Feb 25.
Sunflower (Helianthus annuus) stem canker caused by Diaporthe helianthi is one of the most important sunflower diseases in Croatia. Until recently, sunflower was the only known host for D. helianthi. In our research carried out in the area of Eastern Croatia, isolates of Diaporthe/Phomospis were collected from Xanthium italicum, X. strumarium and Arctium lappa. Using morphological, cultural and molecular ITS rDNA data, isolates from these weeds were identified as D. helianthi. The following isolates were used in the pathogenicity test: one isolate originated from sunflower (Su5/04), three from X. italicum (Xa2, Xa3 and Xa5), two from X. strumarium (Xa9 and Xa12), one from Xanthium sp. (Xa13) and one from A. lappa (Ar3). According to the results, it was determined that isolate Xa5 (originated from X. italicum) was the most pathogenic to sunflower stems. The average length of the lesion was 11.3 cm. The lowest level of pathogenicity was found in Xa9 (isolated from X. strumarium). The length of the lesion was 0.1 cm.
向日葵茎溃疡病由 Helianthus annuus 引起的 Diaporthe helianthi 是克罗地亚最重要的向日葵病害之一。直到最近，向日葵还是 D. helianthi 的唯一已知宿主。在我们在克罗地亚东部地区进行的研究中，从 Xanthium italicum、X. strumarium 和 Arctium lappa 中收集到了 Diaporthe/Phomospis 的分离物。使用形态学、培养和分子 ITS rDNA 数据，这些杂草中的分离物被鉴定为 D. helianthi。以下分离物用于致病性测试：一个分离物来自向日葵（Su5/04），三个来自 X. italicum（Xa2、Xa3 和 Xa5），两个来自 X. strumarium（Xa9 和 Xa12），一个来自 Xanthium sp.（Xa13）和一个来自 A. lappa（Ar3）。根据结果，确定分离物 Xa5（来自 X. italicum）对向日葵茎的致病性最强。病变的平均长度为 11.3 厘米。在 Xa9（分离自 X. strumarium）中发现的致病性最低。病变的长度为 0.1 厘米。
