Zhang Ji-Xia, He Zhi-An, Chen Zheng-Yue, Wang Yong-Xue, Bai Su-Ping, Sun Han-Dong
Pharmaceutical Laboratory, Xinxiang Medical University, Xinxiang, Henan Province, China.
J Asian Nat Prod Res. 2009 Aug;11(8):693-7. doi: 10.1080/10286020802361271.
Two new ent-kaurane diterpenoids, dayecrystals D-E (1-2), together with nine known compounds, isojaponin A (3), rabdosin A (4), lushanrubescensin J (5), wikstroemioidin B (6), maoyecrystal C (7), rabdosin B (8), isodonal (9), shikokianin (10), and effusanin A (11), were isolated from the leaves of Isodon macrophyllus. The structures of the new compounds were elucidated using 1D and 2D NMR spectroscopy. The (13)C-NMR spectral data of compound 4 are reported for the first time. All of the compounds were tested for their cytotoxicities against DU145 and LoVo human tumor cells. Compounds 4, 10, and 11 showed inhibitory effects on DU145 cells with IC(50) values 5.90, 4.24, and 3.16 microM, and LoVo cells with IC(50) values 14.20, 17.55, and 3.02 microM, respectively.
从大叶香茶菜的叶子中分离出两种新的对映 - 贝壳杉烷二萜类化合物，大叶结晶D - E（1 - 2），以及九种已知化合物，异日本皂苷A（3）、香茶菜辛A（4）、庐山香茶菜素J（5）、南蛇藤黄素B（6）、毛叶结晶C（7）、香茶菜辛B（8）、异冬珊瑚碱（9）、日本常山素（10）和散花素A（11）。使用一维和二维核磁共振光谱对新化合物的结构进行了阐明。首次报道了化合物4的碳 - 核磁共振光谱数据。对所有化合物针对DU145和LoVo人肿瘤细胞的细胞毒性进行了测试。化合物4、10和11对DU145细胞显示出抑制作用，其半数抑制浓度（IC50）值分别为5.90、4.24和3.16微摩尔，对LoVo细胞的IC50值分别为14.20、17.55和3.02微摩尔。
