Department of Biochemistry, College of Veterinary Medicine, University of Kafkas, Kars, Turkey.
Eur Rev Med Pharmacol Sci. 2010 Jan;14(1):19-23.
The aim of this study was to determine the effect of exogenous GSH, an important antioxidant containing thiol group, on total antioxidant capacity (TAC) and total oxidant capacity (TOC), adenosine deaminase (ADA), a significant indicator of cellular immunity, and nitric oxide (NO) concentrations in rabbits. Sixteen healthy New Zealand rabbits were divided into 2 groups as control and GSH-treated group. Animals in control group received single intraperitoneal (ip) administration of 0.9% saline. Rabbits in GSH-treated group received reduced L-glutathione (10 mg/kg) (ip). Blood samples were taken from the marginal ear vein 0, 3, 6 and 12 hours after injection. Plasma TAC and NO levels were not statistically different between control and GSH group at 0 and 12 hours. Plasma TAC level was found to be significantly higher (p < 0.05) while NO level was found to be significantly lower (p < 0.05) in GSH treated group when compared to control group at 3 and 6 hours. Plasma TOC and ADA activity were not statistically different between control and GSH group during experiment. In conclusion, exogenous GSH resulted in an alteration of TAC and NO but not TOC and ADA, so exogenous GSH may be a valuable enhancer of the antioxidant system.
本研究旨在确定外源性 GSH（一种含有巯基的重要抗氧化剂）对总抗氧化能力（TAC）和总氧化能力（TOC）、腺苷脱氨酶（ADA）（细胞免疫的重要指标）和一氧化氮（NO）浓度的影响。16 只健康的新西兰兔被分为对照组和 GSH 处理组。对照组动物接受单次腹腔内（ip）注射 0.9%生理盐水。GSH 处理组的兔子接受还原型 L-谷胱甘肽（10mg/kg）（ip）。注射后 0、3、6 和 12 小时从耳缘静脉取血样。在 0 和 12 小时，对照组和 GSH 组之间的血浆 TAC 和 NO 水平没有统计学差异。与对照组相比，在 3 和 6 小时时，GSH 处理组的血浆 TAC 水平显著升高（p < 0.05），而 NO 水平显著降低（p < 0.05）。在实验过程中，对照组和 GSH 组之间的血浆 TOC 和 ADA 活性没有统计学差异。总之，外源性 GSH 导致 TAC 和 NO 发生变化，但 TOC 和 ADA 没有变化，因此外源性 GSH 可能是抗氧化系统的有价值的增强剂。
