Institute of Forestry, Pokhara Campus, Tribhuvan University, Pokhara, Kaski, Nepal.
Planta Med. 2010 Jul;76(10):1022-5. doi: 10.1055/s-0029-1240896. Epub 2010 Feb 25.
Four new 5 alpha-pregnane-type steroidal alkaloids, hookerianamides L(1), M(2), N(3), and O(4), and a known N-formylchonemorphine (5) have been isolated by acid-base extraction of the dichloromethane extract of Sarcococca hookeriana. The structures of all compounds were determined with spectroscopic techniques and by comparison with literature data. All compounds displayed antileishmanial and antibacterial properties. Compounds 1, 4, and 5 were found to be more potent than standard pentamidine (IC (50) = 9.59 microg/mL) with respect to leishmanicidal activity. The minimum inhibitory concentration of most of the compounds against Bacillus subtilis, Streptococcus minor, and Streptococcus ferus was lower than that of the standard ampicillin.
从钩腺崖爬藤的二氯甲烷提取物中经酸碱提取，分离得到四个新的 5α-孕甾烷型甾体生物碱，钩藤酰胺 L（1）、M（2）、N（3）和 O（4），以及一个已知的 N-甲酰基去氢吗啡碱（5）。通过光谱技术和与文献数据的比较确定了所有化合物的结构。所有化合物均表现出抗利什曼原虫和抗菌活性。化合物 1、4 和 5 在杀利什曼原虫活性方面比标准戊烷脒（IC（50）=9.59μg/mL）更有效。大多数化合物对枯草芽孢杆菌、小链球菌和发酵链球菌的最小抑菌浓度均低于标准氨苄青霉素。
