从. 中分离得到的两种新型细胞毒性甾体生物碱。
Two New Cytotoxic Steroidal Alkaloids from .
机构信息
Guiyang College of Traditional Chinese Medicine, Guiyang 550025, China.
Guizhou Provincial Key Laboratory of Miao Medicine, Guiyang 550025, China.
出版信息
Molecules. 2018 Dec 20;24(1):11. doi: 10.3390/molecules24010011.
Two new steroidal alkaloids, named hookerianine A () and hookerianine B () were isolated from the stems and roots of Baill., along with two known compounds, sarcorucinine G () and epipachysamine D (). On the basis of spectroscopic methods and by comparison with literature data, their structures were determined. As well as X-ray crystallography was performed to confirm compound . To identify novel antitumor inhibitors, all compounds were performed a CCK-8 assay against five human cancer cell lines SW480, SMMC-7721, PC3, MCF-7 and K562 in vitro. Compound exhibited moderate cytotoxic activities to all cell lines with IC values in the range of 5.97⁻19.44 μM. Compound was the most effective one against SW480 and K562 cell lines with IC values of 5.77 and 6.29 μM, respectively.
从 Baill. 的茎和根中分离得到两种新的甾体生物碱，命名为钩藤碱 A () 和钩藤碱 B ()，以及两种已知化合物，桑根皮定 G () 和表育嗪胺 D ()。基于光谱方法并与文献数据进行比较，确定了它们的结构。还进行了 X 射线晶体学以确认化合物 。为了鉴定新型抗肿瘤抑制剂，对所有化合物进行了 CCK-8 测定，以评估它们对体外五种人癌细胞系 SW480、SMMC-7721、PC3、MCF-7 和 K562 的抑制活性。化合物 对所有细胞系均表现出中等的细胞毒性，IC 值范围为 5.97⁻19.44 μM。化合物 对 SW480 和 K562 细胞系的抑制活性最强，IC 值分别为 5.77 和 6.29 μM。
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