一种从食用野生蘑菇红绒盖牛肝菌中提取的具有高效抗增殖和 HIV-1 逆转录酶抑制活性的新型凝集素。
A novel lectin with highly potent antiproliferative and HIV-1 reverse transcriptase inhibitory activities from the edible wild mushroom Russula delica.
机构信息
State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing 100094, China.
出版信息
Glycoconj J. 2010 Feb;27(2):259-65. doi: 10.1007/s10719-009-9274-5.
A dimeric lectin with a molecular weight of 60 kDa and high hemagglutinating activity was isolated from fresh fruiting bodies of the wild mushroom Russula delica. The lectin was composed of two identical subunits, each with a molecular weight of 30 kDa. It was adsorbed on both SP-Sepharose and Q-Sepharose and unadsorbed on DEAE-cellulose. Its hemagglutinating activity was stable up to 70 degrees C, and in HCl and NaOH solutions of concentrations up to 25 and 12.5 mM, respectively. The activity was inhibited by inulin and o-nitrophenyl-beta-D-galactopyranoside. Al3+, Fe3+ and Zn2+ ions, but not by Ca2+, Mg2+ and Mn2+ ions. Mg2+ ions at 10 mM concentration potentiated the hemagglutinating activity of the lectin. Russula delica lectin was devoid of mitogenic activity toward mouse splenocytes, but potently inhibited proliferation of HepG2 hepatoma and MCF 7 breast cancer cells, with an IC50 value of 0.88 microM and 0.52 microM, respectively. It potently inhibited HIV-1 reverse transcriptase activity with an IC50 of 0.26 microM.
从野生蘑菇红绒盖牛肝菌新鲜子实体中分离得到一种分子量为 60 kDa、具有高血凝活性的二聚凝集素。该凝集素由两个相同的亚基组成，每个亚基分子量为 30 kDa。它可以被 SP-Sepharose 和 Q-Sepharose 吸附，但不能被 DEAE-纤维素吸附。其血凝活性在 70°C 下稳定，在浓度高达 25 和 12.5 mM 的 HCl 和 NaOH 溶液中稳定。该活性被菊粉和邻硝基苯-β-D-半乳糖吡喃糖苷抑制。Al3+、Fe3+和 Zn2+离子，但不是 Ca2+、Mg2+和 Mn2+离子。10 mM 浓度的 Mg2+离子增强了凝集素的血凝活性。红绒盖牛肝菌凝集素对小鼠脾细胞没有有丝分裂活性，但能强烈抑制 HepG2 肝癌和 MCF 7 乳腺癌细胞的增殖，IC50 值分别为 0.88 μM 和 0.52 μM。它强烈抑制 HIV-1 逆转录酶活性，IC50 值为 0.26 μM。
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