Clinical Chemistry, Erasmus MC, University Medical Center, Rotterdam, The Netherlands.
Clin Chem Lab Med. 2010 May;48(5):665-75. doi: 10.1515/CCLM.2010.108.
We evaluated the body fluid (BF) mode on the new Sysmex XE-5000 analyzer.
Red (RBC) and white blood cell (WBC) (differential) counts of BFs (139 patient samples and 87 normal samples) were measured and compared to the Fuchs-Rosenthal chamber and stained cytospin slides.
Extended cell counting using the BF mode was noted to have an improved WBC detection limit (CV(20)%) of 10 x 10(6)/L. Excellent agreement with the manual method was observed for most BFs [mean bias +2 to 6 x 10(6)/L for cerebrospinal fluid (CSF) and -1 to 12 x 10(6)/L for other fluids]. In CSF, the BF-mode counted more WBC (polymorphic nuclear cells) compared with the manual method (mean bias +5 to 6 x 10(6)/L), especially in samples with low cell counts (<20 x 10(6)/L). Carry over was negligible (mostly <0.17%) and linearity was excellent (mean bias <5%). The reference ranges for CSF (n=87) were RBC 0 x 10(6)/L, WBC and mononuclear <7 x 10(6)/L, and polymorph nucleated cells <3 x 10(6)/L.
The BF mode on the Sysmex XE-5000 offers rapid and accurate RBC and WBC (differential) counts in clinically relevant concentration ranges in CSF and other fluids. In addition, the exclusion of high fluorescent cells, such as mesothelial cells and macrophages from WBC counting may reduce the number of manual analyses in pleural fluids and ascites.
我们评估了新的 Sysmex XE-5000 分析仪上的体液（BF）模式。
测量了 BF（139 例患者样本和 87 例正常样本）的红细胞（RBC）和白细胞（WBC）（差异）计数，并与 Fuchs-Rosenthal 室和染色细胞旋片进行比较。
使用 BF 模式进行扩展细胞计数，WBC 的检测限（CV（20）%）提高到 10 x 10（6）/L。对于大多数 BF，与手动方法的一致性非常好[脑脊液（CSF）的平均偏差为+2 到 6 x 10（6）/L，其他液体为-1 到 12 x 10（6）/L]。在 CSF 中，BF 模式计数的 WBC（多形核细胞）比手动方法多（平均偏差+5 到 6 x 10（6）/L），尤其是在细胞计数较低（<20 x 10（6）/L）的样本中。携带物可以忽略不计（大多<0.17%），线性度非常好（平均偏差<5%）。CSF 的参考范围（n=87）为 RBC 0 x 10（6）/L，WBC 和单核细胞<7 x 10（6）/L，多形核细胞<3 x 10（6）/L。
Sysmex XE-5000 上的 BF 模式在 CSF 和其他液体的临床相关浓度范围内提供了快速准确的 RBC 和 WBC（差异）计数。此外，排除 WBC 计数中的高荧光细胞，如间皮细胞和巨噬细胞，可能会减少胸腔积液和腹水的手动分析数量。
