Ferguson B M, Brewer B J, Reynolds A E, Fangman W L
Department of Genetics SK-50, University of Washington, Seattle 98195.
Cell. 1991 May 3;65(3):507-15. doi: 10.1016/0092-8674(91)90468-e.
The mechanism that causes large regions of eukaryotic chromosomes to remain unreplicated until late in S phase is not understood. We have found that 67 kb of telomere-adjacent DNA at the right end of chromosome V in S. cerevisiae is replicated late in S phase. An ARS element in this region, ARS501, was shown by two-dimensional gel analysis to be an active origin of replication. Kinetic analyses indicate that the rate of replication fork movement within this late region is similar to that in early replicating regions. Therefore, the delayed replication of the region is a consequence of late origin activation. The results also support the idea that the pattern of interspersed early and late replication along the chromosomes of higher eukaryotes is a consequence of the temporal regulation of origin activation.
导致真核生物染色体大片段直到S期晚期才进行复制的机制尚不清楚。我们发现，酿酒酵母V号染色体右端67 kb的端粒相邻DNA在S期晚期进行复制。通过二维凝胶分析表明，该区域的一个ARS元件ARS501是一个活跃的复制起点。动力学分析表明，该晚期区域内复制叉移动的速率与早期复制区域相似。因此，该区域复制延迟是晚期起点激活的结果。这些结果也支持这样一种观点，即高等真核生物染色体上早期和晚期复制相间的模式是起点激活时间调控的结果。
